Lu L J, Kurup V P, Hoffman D R, Kelly K J, Murali P S, Fink J N
Department of Medicine, Medical College of Wisconsin, Milwaukee 53295, USA.
J Immunol. 1995 Sep 1;155(5):2721-8.
Hypersensitivity to latex proteins present in health care products, particularly in latex gloves, has emerged as an important public health problem in recent years. Most of these latex allergens have not been purified or characterized. Here we report the purification and characterization of a 23-kDa latex polypeptide from nonammoniated natural rubber latex. This purified 23-kDa polypeptide reacted with IgE from 13 of 17 (76%) latex-allergic spina bifida patients, but from only 1 of 5 health care workers with latex allergy. Furthermore, all the sera of patients with anaphylaxis to latex showed reactivity with the 23-kDa polypeptide. This allergen also induced significant lymphocyte proliferation by PBMC from spina bifida patients, but not from health care workers. The 90 amino acid residues sequenced from the 23-kDa polypeptide showed a 45.6% homology with a previously reported latex protein, rubber elongation factor. By using a mAb LAM-1, produced against the 23-kDa allergen, we could also detect this allergen or its epitope in ammoniated latex and natural rubber latex products used in health care management, but not in non-latex extracts studied.
近年来，对医疗保健产品中存在的乳胶蛋白（尤其是乳胶手套中的乳胶蛋白）过敏已成为一个重要的公共卫生问题。这些乳胶过敏原大多尚未得到纯化或鉴定。在此，我们报告了从非氨化天然橡胶乳胶中纯化和鉴定出一种23 kDa的乳胶多肽。这种纯化的23 kDa多肽与17名乳胶过敏脊柱裂患者中的13名（76%）的IgE发生反应，但在5名乳胶过敏医护人员中只有1人有反应。此外，所有对乳胶过敏反应的患者血清均与23 kDa多肽发生反应。这种过敏原还能诱导脊柱裂患者的外周血单个核细胞（PBMC）显著增殖，但医护人员的PBMC则无此反应。从23 kDa多肽中测序得到的90个氨基酸残基与先前报道的乳胶蛋白橡胶延伸因子有45.6%的同源性。通过使用针对23 kDa过敏原产生的单克隆抗体LAM-1，我们还能在用于医疗管理的氨化乳胶和天然橡胶乳胶产品中检测到这种过敏原或其表位，但在所研究的非乳胶提取物中未检测到。
