Kurup V P, Yeang H Y, Sussman G L, Bansal N K, Beezhold D H, Kelly K J, Hoffman D R, Williams B, Fink J N
Department of Medicine, Allergy-Immunology Division, Medical College of Wisconsin and Research Service, Veterans Affairs Medical Center, Milwaukee, WI 53295, USA.
Clin Exp Allergy. 2000 Mar;30(3):359-69. doi: 10.1046/j.1365-2222.2000.00748.x.
Latex allergy is largely an occupational allergy due to sensitization to natural rubber latex allergens present in a number of health care and household products. Although several purified allergens are currently available for study, information on the usefulness of these purified, native or recombinant allergens in the demonstration of specific immunoglobulin (Ig) E in the sera of patients is lacking.
To evaluate the purified latex allergens and to demonstrate specific IgE antibody in the sera of health care workers and spina bifida patients with clinical latex allergy.
Two radioallergosorbent and an enzyme-linked immunosorbent assay (ELISA) using latex proteins Hev b 1, 2, 3, 4, 6 and 7 along with two glove extracts and Malaysian nonammoniated latex (MNA) were evaluated to demonstrate IgE in the sera of health care workers and spina bifida with latex allergy and controls with no history of latex allergy.
ELISA using the purified latex allergens demonstrated specific IgE in 32-65% health care workers and 54-100% of spina bifida patients with latex allergy. The corresponding figures for RAST were 13-48 and 23-85 for RAST-1 and 19-61 and 36-57 for RAST-2. These results were comparable with the results obtained with glove extracts and crude rubber latex proteins.
When used simultaneously, latex proteins Hev b 2 and Hev b 7 reacted significantly with specific serum IgE in 80% of health care workers and 92% of spina bifida patients with latex allergy by ELISA technique, while this combination gave lower positivity when the RASTs were used. By the addition of Hev b 3, specific IgE was detected in all spina bifida patients with latex allergy. Both RASTs failed to show specific IgE in the control subjects, while the ELISA showed significant latex-specific IgE in 22% of controls.
乳胶过敏很大程度上是一种职业性过敏，是由于对许多医疗保健和家用产品中存在的天然橡胶乳胶过敏原致敏所致。尽管目前有几种纯化的过敏原可供研究，但关于这些纯化的、天然的或重组的过敏原在检测患者血清中特异性免疫球蛋白（Ig）E方面的有用性的信息却很缺乏。
评估纯化的乳胶过敏原，并在有临床乳胶过敏的医护人员和脊柱裂患者血清中检测特异性IgE抗体。
评估了两种放射变应原吸附试验和一种酶联免疫吸附测定（ELISA），使用乳胶蛋白Hev b 1、2、3、4、6和7以及两种手套提取物和马来西亚非氨化乳胶（MNA），以检测有乳胶过敏的医护人员和脊柱裂患者以及无乳胶过敏史的对照者血清中的IgE。
使用纯化乳胶过敏原的ELISA在32% - 65%的医护人员和54% - 100%有乳胶过敏的脊柱裂患者中检测到特异性IgE。RAST - 1的相应数字为13% - 48%和23% - 85%，RAST - 2的相应数字为19% - 61%和36% - 57%。这些结果与使用手套提取物和粗橡胶乳胶蛋白获得的结果相当。
同时使用时，乳胶蛋白Hev b 2和Hev b 7通过ELISA技术在80%的医护人员和92%有乳胶过敏的脊柱裂患者中与特异性血清IgE有显著反应，而使用RAST时该组合的阳性率较低。加入Hev b 3后，在所有有乳胶过敏的脊柱裂患者中均检测到特异性IgE。两种RAST在对照受试者中均未显示特异性IgE，而ELISA在22%的对照者中显示出显著的乳胶特异性IgE。
