Fujiwara K, Mochida S, Nagoshi S, Iijima O, Matsuzaki Y, Takeda S, Aburada M
Third Department of Internal Medicine, Saitama Medical School, Japan.
J Ethnopharmacol. 1995 May;46(2):107-14. doi: 10.1016/0378-8741(95)01235-6.
The effect of Xiao-Chai-Hu-Tang (Sho-saiko-to, TJ-9), the extract of a mixture of 7 herbs, on hepatic macrophage function was studied using rats. Hepatic macrophages were activated by injection of Corynebacterium parvum or 70% partial hepatectomy. Oral administration of TJ-9 for 3 weeks did not affect the ability of these macrophages to produce superoxide anions evaluated in situ by liver perfusion with nitro blue tetrazolium (NBT) and phorbol myristate acetate (PMA). However, the similar administration of TJ-9 attenuated the blocking of the activation after partial hepatectomy produced by pretreatment with gum arabic, a polysaccharide of high molecular weight. When gum arabic was added to the medium of rat hepatic macrophages cultured with normal rat sera, their ability to produce superoxide anions was reduced in a dose-related manner. This reduction was attenuated by changing the sera to the sera obtained from rats given oral doses of TJ-9 for 3 weeks. These results suggest that TJ-9 may improve the blocked function of hepatic macrophages in activation.
采用大鼠研究了由7种草药混合而成的小柴胡汤提取物（TJ-9）对肝巨噬细胞功能的影响。通过注射短小棒状杆菌或进行70%部分肝切除术来激活肝巨噬细胞。连续3周口服TJ-9对这些巨噬细胞产生超氧阴离子的能力没有影响，超氧阴离子的产生通过用硝基蓝四氮唑（NBT）和佛波醇肉豆蔻酸酯乙酸盐（PMA）进行肝脏灌注在原位进行评估。然而，类似的TJ-9给药减弱了由阿拉伯胶（一种高分子量多糖）预处理导致的部分肝切除术后激活的阻断作用。当将阿拉伯胶添加到用正常大鼠血清培养的大鼠肝巨噬细胞培养基中时，它们产生超氧阴离子的能力以剂量相关的方式降低。将血清换成来自连续3周口服TJ-9的大鼠的血清后，这种降低作用减弱。这些结果表明TJ-9可能改善肝巨噬细胞激活中被阻断的功能。
