Ohta Y, Nishida K, Sasaki E, Kongo M, Hayashi T, Nagata M, Ishiguro I
Department of Biochemistry, School of Medicine, Fujita Health University, Aichi, Japan.
Am J Chin Med. 1997;25(3-4):333-42. doi: 10.1142/S0192415X97000378.
The preventive effect of Sho-saiko-to (Xiao-Chaihu-Tang) extract (TJ-9) on the progression of D-galactosamine (GaIN)-induced liver injury was examined in five week-old male Wistar rats with oral (p.o.) or intraperitoneal (i.p.) administration of the same dose of TJ-9. Rats treated once with GaIN (500 mg/kg body weight, i.p.) received TJ-9 at a dose of 1.0 g/kg body weight (p.o. or i.p.) 2 hours after GaIN treatment at which time an apparent liver injury occurred. Both p.o. and i.p. administration of TJ-9 showed similar significant prevention against the progression of liver injury 24 hours after GaIN injection. Although total protein and albumin concentrations in serum and protein concentration in the liver decreased with the progression of GaIN-induced liver injury, oral or i.p. administration of TJ-9 prevented these decreases in similar degree. However, decreases in serum and liver triglyceride concentration with the progression of liver injury were not attenuated after p.o. or i.p. administration of TJ-9. The activities of liver 5'-nucleotidase and glucose-6-phosphatase, marker enzymes of liver plasma and microsomal membranes, respectively, decreased during the progression of liver injury. A similar preventive effect on the decrease of both enzyme activities was found after p.o. or i.p. administration of TJ-9. These results indicate that the preventive effect on progression of GaIN-induced liver injury by oral or i.p. administration is approximately equal, and that the effect may be through improving the impaired liver protein synthesis and disrupted liver plasma and microsomal membranes in a similar degree.
在5周龄雄性Wistar大鼠中，通过口服（p.o.）或腹腔注射（i.p.）相同剂量的小柴胡汤提取物（TJ - 9），研究其对D - 半乳糖胺（GalN）诱导的肝损伤进展的预防作用。用GalN（500 mg/kg体重，腹腔注射）单次处理的大鼠在GalN处理2小时后接受剂量为1.0 g/kg体重的TJ - 9（口服或腹腔注射），此时明显的肝损伤已经发生。在GalN注射24小时后，口服和腹腔注射TJ - 9均显示出对肝损伤进展的相似显著预防作用。尽管随着GalN诱导的肝损伤进展，血清中的总蛋白和白蛋白浓度以及肝脏中的蛋白浓度降低，但口服或腹腔注射TJ - 9在相似程度上预防了这些降低。然而，口服或腹腔注射TJ - 9后，随着肝损伤进展血清和肝脏甘油三酯浓度的降低并未减弱。肝损伤进展过程中，分别作为肝细胞膜和微粒体膜标记酶的肝脏5'-核苷酸酶和葡萄糖-6-磷酸酶的活性降低。口服或腹腔注射TJ - 9后，对这两种酶活性的降低发现了相似的预防作用。这些结果表明，口服或腹腔注射对GalN诱导的肝损伤进展的预防作用大致相等，并且该作用可能是通过相似程度地改善受损的肝脏蛋白质合成以及破坏的肝细胞膜和微粒体膜来实现的。
