Ohta Y, Sasaki E, Nishida K, Kobayashi T, Nagata M, Ishiguro I
Department of Biochemistry, School of Medicine, Fujita Health University, Japan.
Am J Chin Med. 1995;23(1):53-64. doi: 10.1142/S0192415X95000080.
In order to clarify the preventive action of Dai-Saiko-to (Da-Chai-Hu-Tang) extract (TJ-8) on the progression of acute liver injury in rats intoxicated with carbon tetrachloride (CCl4), we examined the effect of post-oral TJ-8 administration on hepatic active oxygen metabolism following the progression of this liver damage. When TJ-8 (1.0 g/kg body weight) was administered orally to male Wistar rats aged five weeks 2 hrs after i.p. injection of CCl4 (1.0 ml/kg body weight), an apparent liver injury occurred. Significant prevention against the progression of liver injury was found at 24 hrs after injection, judging from the activities of serum transaminases, indexes of liver cell damage. Liver cytosolic superoxide dismutase (SOD) activity decreased 2 and 24 hrs after CCl4 injection, while liver cytosolic catalase and glutathione reductase (GSSG-R) activities decreased 24 hrs after the injection. At 2 and 24 hrs after CCl4 treatment, liver cytosolic Se-containing glutathione peroxidase (GSH-px) activity did not change and liver cytosolic glucose-6-phosphate dehydrogenase (G-6-PDH) activity increased. Post-oral TJ-8 administration significantly ameliorated decreases in liver SOD, catalase, and GSSG-R activities at 24 hrs after CCl4 injection, but did not affect liver Se-GSH-px and increased liver G-6-PDH activities at 24 hrs after the injection. Although increased liver lipid peroxide level and decreased liver reduced glutathione and ascorbic acid levels were observed 2 and 24 hrs after CCl4 injection, post-oral TJ-8 administration significantly prevented these changes found at 24 hrs after injection. These results indicate that post-oral TJ-8 administration can prevent the progression of acute liver injury in CCl4-injected rats by inhibiting enhanced lipid peroxidation and by improving disrupted active oxygen metabolism in the injured liver.
为阐明柴芍承气汤提取物（TJ - 8）对四氯化碳（CCl4）中毒大鼠急性肝损伤进展的预防作用，我们在肝损伤进展后，研究了口服TJ - 8对肝脏活性氧代谢的影响。将体重1.0 ml/kg的CCl4腹腔注射给5周龄雄性Wistar大鼠2小时后，口服TJ - 8（1.0 g/kg体重），出现明显肝损伤。从血清转氨酶活性（肝细胞损伤指标）判断，注射后24小时发现对肝损伤进展有显著预防作用。CCl4注射后2小时和24小时，肝脏胞质超氧化物歧化酶（SOD）活性降低，而肝脏胞质过氧化氢酶和谷胱甘肽还原酶（GSSG - R）活性在注射后24小时降低。CCl4处理后2小时和24小时，肝脏胞质含硒谷胱甘肽过氧化物酶（GSH - px）活性未改变，肝脏胞质葡萄糖 - 6 - 磷酸脱氢酶（G - 6 - PDH）活性增加。口服TJ - 8后显著改善了CCl4注射后24小时肝脏SOD、过氧化氢酶和GSSG - R活性的降低，但不影响注射后24小时肝脏硒 - GSH - px活性，也不影响肝脏G - 6 - PDH活性的增加。虽然CCl4注射后2小时和24小时观察到肝脏脂质过氧化物水平升高，肝脏还原型谷胱甘肽和抗坏血酸水平降低，但口服TJ - 8后显著预防了注射后24小时出现的这些变化。这些结果表明，口服TJ - 8可通过抑制脂质过氧化增强和改善受损肝脏中活性氧代谢紊乱来预防CCl4注射大鼠急性肝损伤的进展。
