Ma C, Rupert C S
Program in Molecular and Cell Biology, University of Texas at Dallas, Richardson 75083-0688, USA.
Mol Gen Genet. 1995 Jul 22;248(1):52-8. doi: 10.1007/BF02456613.
We have identified two promoters of the Escherichia coli phr gene by DNA deletion mapping, S1 mapping of transcripts and sequence homology. The weaker promoter, P2, located approximately 530 bp upstream from the start codon, extends beyond the previously known nucleotide sequence. The stronger, P1, lies 90 bp from the gene and is distinct from three previously described promoter-like sequences nearby. beta-Galactosidase production from a plasmid-borne gene, promoted by a synthetic copy of P1, increases after DNA damage, but the increase does not depend on the SOS-box-like sequences normally present in the vicinity of P1. Induction still requires intact recA and lexA genes, and also intact sulA.
我们通过DNA缺失图谱、转录本的S1图谱分析及序列同源性鉴定出了大肠杆菌phr基因的两个启动子。较弱的启动子P2位于起始密码子上游约530 bp处，延伸至先前已知的核苷酸序列之外。较强的启动子P1距离该基因90 bp，且与附近先前描述的三个启动子样序列不同。由P1的合成拷贝促进的质粒携带基因产生的β-半乳糖苷酶，在DNA损伤后增加，但这种增加并不依赖于通常存在于P1附近的SOS盒样序列。诱导仍需要完整的recA和lexA基因，以及完整的sulA基因。
