枯草芽孢杆菌中用于克隆启动子的载体构建
Construction of a vector for cloning promoters in Bacillus subtilis.
作者信息
Band L, Yansura D G, Henner D J
出版信息
Gene. 1983 Dec;26(2-3):313-5. doi: 10.1016/0378-1119(83)90204-4.
PMID:6323271
Abstract
A versatile vector for cloning DNA fragments containing promoter activity in Bacillus subtilis was derived from plasmids pBR322, pUB110 and pC194. Selection is based on chloramphenicol resistance which is dependent upon the introduction of DNA fragments allowing expression of a chloramphenicol acetyl transferase gene. The plasmid contains a second selectable marker, neomycin resistance, and contains functional origins of replication for both B. subtilis and Escherichia coli.
摘要
一种用于克隆含有枯草芽孢杆菌启动子活性DNA片段的多功能载体，源自质粒pBR322、pUB110和pC194。选择基于氯霉素抗性，这取决于引入允许氯霉素乙酰转移酶基因表达的DNA片段。该质粒含有第二个选择标记——新霉素抗性，并且含有枯草芽孢杆菌和大肠杆菌的功能性复制起点。
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