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Genetic and biochemical interactions between an essential kinetochore protein, Cbf2p/Ndc10p, and the CDC34 ubiquitin-conjugating enzyme.一种重要的动粒蛋白Cbf2p/Ndc10p与CDC34泛素结合酶之间的遗传和生化相互作用。
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2
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Cell cycle arrest in cdc20 mutants of Saccharomyces cerevisiae is independent of Ndc10p and kinetochore function but requires a subset of spindle checkpoint genes.酿酒酵母cdc20突变体中的细胞周期停滞不依赖于Ndc10p和动粒功能，但需要纺锤体检查点基因的一个子集。
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10
Suppressors of the ndc10-2 mutation: a role for the ubiquitin system in Saccharomyces cerevisiae kinetochore function.ndc10 - 2突变的抑制因子：泛素系统在酿酒酵母动粒功能中的作用
Genetics. 1997 Oct;147(2):409-20. doi: 10.1093/genetics/147.2.409.

本文引用的文献

1
Identification of essential components of the S. cerevisiae kinetochore.酿酒酵母动粒关键组分的鉴定。
Cell. 1993 May 21;73(4):761-74. doi: 10.1016/0092-8674(93)90255-o.
2
Isolation and characterization of a gene (CBF2) specifying a protein component of the budding yeast kinetochore.一种指定芽殖酵母动粒蛋白成分的基因（CBF2）的分离与特性分析。
J Cell Biol. 1993 May;121(3):513-9. doi: 10.1083/jcb.121.3.513.
3
NDC10: a gene involved in chromosome segregation in Saccharomyces cerevisiae.NDC10：酿酒酵母中一个参与染色体分离的基因。
J Cell Biol. 1993 May;121(3):503-12. doi: 10.1083/jcb.121.3.503.
4
An inhibitor of p34CDC28 protein kinase activity from Saccharomyces cerevisiae.来自酿酒酵母的p34CDC28蛋白激酶活性抑制剂。
Science. 1993 Jan 8;259(5092):216-9. doi: 10.1126/science.8421781.
5
Multiple ubiquitin-conjugating enzymes participate in the in vivo degradation of the yeast MAT alpha 2 repressor.多种泛素结合酶参与酵母MATα2阻遏物的体内降解。
Cell. 1993 Jul 30;74(2):357-69. doi: 10.1016/0092-8674(93)90426-q.
6
The bacterially expressed yeast CDC34 gene product can undergo autoubiquitination to form a multiubiquitin chain-linked protein.细菌表达的酵母CDC34基因产物可进行自身泛素化，形成多聚泛素链连接的蛋白质。
J Biol Chem. 1993 Mar 15;268(8):5668-75.
7
The Ubc3 (Cdc34) ubiquitin-conjugating enzyme is ubiquitinated and phosphorylated in vivo.泛素结合酶Ubc3（Cdc34）在体内发生泛素化和磷酸化。
Mol Cell Biol. 1994 May;14(5):3022-9. doi: 10.1128/mcb.14.5.3022-3029.1994.
8
KAR3-encoded kinesin is a minus-end-directed motor that functions with centromere binding proteins (CBF3) on an in vitro yeast kinetochore.由KAR3编码的驱动蛋白是一种向负端移动的分子马达，它与体外酵母动粒上的着丝粒结合蛋白（CBF3）共同发挥作用。
Proc Natl Acad Sci U S A. 1994 Jul 19;91(15):7212-6. doi: 10.1073/pnas.91.15.7212.
9
Factors required for the binding of reassembled yeast kinetochores to microtubules in vitro.体外重组酵母动粒与微管结合所需的因子。
J Cell Biol. 1994 Nov;127(4):995-1008. doi: 10.1083/jcb.127.4.995.
10
A zinc finger protein, essential for chromosome segregation, constitutes a putative DNA binding subunit of the Saccharomyces cerevisiae kinetochore complex, Cbf3.一种对染色体分离至关重要的锌指蛋白，构成了酿酒酵母动粒复合体Cbf3的一个假定DNA结合亚基。
EMBO J. 1994 Nov 1;13(21):5203-11. doi: 10.1002/j.1460-2075.1994.tb06851.x.

一种重要的动粒蛋白Cbf2p/Ndc10p与CDC34泛素结合酶之间的遗传和生化相互作用。

Genetic and biochemical interactions between an essential kinetochore protein, Cbf2p/Ndc10p, and the CDC34 ubiquitin-conjugating enzyme.

作者信息

Yoon H J, Carbon J

机构信息

Department of Biological Sciences, University of California, Santa Barbara 93106, USA.

出版信息

Mol Cell Biol. 1995 Sep;15(9):4835-42. doi: 10.1128/MCB.15.9.4835.

DOI:10.1128/MCB.15.9.4835
PMID:7651401
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC230728/
Abstract

CBF2/NDC10/CTF14 encodes the 110-kDa subunit of CBF3, a key component of the yeast centromere/kinetochore. Overexpression of yeast CDC34 specifically suppresses the temperature-sensitive growth phenotype of the ndc10-1 mutation. Mutations in CDC34, which specifies a ubiquitin-conjugating enzyme, arrest yeast cells in the G1 phase of the cell cycle, with no intact spindles formed (M. G. Goebl, J. Yochem, S. Jentsch, J. P. McGrath, A. Varshavsky, and B. Byers, Science 241:1331-1335, 1988). The cdc34-2 mutation drastically alters the pattern of Cbf2p modification. Results of experiments using antibodies against Cbf2p and ubiquitin indicate that Cbf2p is ubiquitinated in vivo. Purified Cdc34p catalyzes the formation of Cbf2p-monoubiquitin conjugate in vitro. These data suggest that Cbf2p is an endogenous substrate of the CDC34 ubiquitin-conjugating enzyme and imply that ubiquitination of a kinetochore protein plays a regulatory role in kinetochore function.

摘要

CBF2/NDC10/CTF14编码CBF3的110 kDa亚基，CBF3是酵母着丝粒/动粒的关键组成部分。酵母CDC34的过表达特异性抑制ndc10 - 1突变的温度敏感生长表型。CDC34中的突变（其编码一种泛素缀合酶）使酵母细胞停滞在细胞周期的G1期，未形成完整的纺锤体（M. G. 戈布尔、J. 约切姆、S. 延奇、J. P. 麦格拉思、A. 瓦尔沙夫斯基和B. 拜尔斯，《科学》241:1331 - 1335，1988年）。cdc34 - 2突变极大地改变了Cbf2p修饰模式。使用抗Cbf2p和泛素抗体的实验结果表明，Cbf2p在体内被泛素化。纯化的Cdc34p在体外催化Cbf2p - 单泛素缀合物的形成。这些数据表明Cbf2p是CDC34泛素缀合酶的内源性底物，并暗示动粒蛋白的泛素化在动粒功能中起调节作用。