Jiang W, Lechner J, Carbon J
Department of Biological Sciences, University of California, Santa Barbara 93106.
J Cell Biol. 1993 May;121(3):513-9. doi: 10.1083/jcb.121.3.513.
We have cloned and determined the nucleotide sequence of the gene (CBF2) specifying the large (110 kD) subunit of the 240-kD multisubunit yeast centromere binding factor CBF3, which binds selectively in vitro to yeast centromere DNA and contains a minus end-directed microtubule motor activity. The deduced amino acid sequence of CBF2p shows no sequence homologies with known molecular motors, although a consensus nucleotide binding site is present. The CBF2 gene is essential for viability of yeast and is identical to NDC10, in which a conditional mutation leads to a defect in chromosome segregation (Goh, P.-Y., and J. V. Kilmartin, in this issue of The Journal of Cell Biology). The combined in vitro and in vivo evidence indicate that CBF2p is a key component of the budding yeast kinetochore.
我们已经克隆并测定了指定240-kD多亚基酵母着丝粒结合因子CBF3的大（110 kD）亚基的基因（CBF2）的核苷酸序列，该因子在体外选择性地结合酵母着丝粒DNA，并具有负端定向微管马达活性。虽然存在一个共有核苷酸结合位点，但CBF2p推导的氨基酸序列与已知分子马达没有序列同源性。CBF2基因对酵母的生存能力至关重要，并且与NDC10相同，其中条件性突变导致染色体分离缺陷（Goh，P.-Y.和J.V. Kilmartin，本期《细胞生物学杂志》）。体外和体内的综合证据表明，CBF2p是出芽酵母动粒的关键成分。
