转录因子Sox-18的反式激活和DNA结合特性
Trans-activation and DNA-binding properties of the transcription factor, Sox-18.
作者信息
Hosking B M, Muscat G E, Koopman P A, Dowhan D H, Dunn T L
机构信息
Centre for Molecular and Cellular Biology, University of Queensland, St Lucia, Australia.
出版信息
Nucleic Acids Res. 1995 Jul 25;23(14):2626-8. doi: 10.1093/nar/23.14.2626.
Sox-18 is a member of the Sox multi-gene family (Sry-related HMG-box gene). We have bacterially expressed this 378 amino acid protein and demonstrated sequence-specific binding to the Sox DNA-binding motif AACAAAG. A distinct 95 amino acid activation domain was mapped in Sox-18 using GAL4-Sox-18 fusions (amino acids 160-225). Furthermore, Sox-18 was capable of trans-activating gene expression through the AACAAA motif. Our results suggest that Sox-18 functions as a classical trans-activator of gene expression.
Sox-18是Sox多基因家族（与性别决定区Y相关的高迁移率族盒基因）的成员。我们已通过细菌表达了这种含378个氨基酸的蛋白质，并证明其能与Sox DNA结合基序AACAAAG进行序列特异性结合。利用GAL4-Sox-18融合蛋白（氨基酸160 - 225）在Sox-18中定位了一个独特的含95个氨基酸的激活结构域。此外，Sox-18能够通过AACAAA基序反式激活基因表达。我们的结果表明，Sox-18作为一种典型的基因表达反式激活因子发挥作用。
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