Jenny A, Keller W
Department of Cell Biology, Biozentrum, University of Basel, Switzerland.
Nucleic Acids Res. 1995 Jul 25;23(14):2629-35. doi: 10.1093/nar/23.14.2629.
3'-processing of mRNA precursors depends on several protein factors. One of them, cleavage and polyadenylation specificity factor (CPSF) is required for the cleavage of the mRNA precursor or as well as for the tail elongation reaction. We have obtained complementary DNA encoding the 160 kDa subunit, which had previously been shown to interact with the AAUAAA polyadenylation signal. The cDNAs code for an open reading frame of 1444 amino acids. The translated protein has a calculated molecular weight of 161 kDa and a predicted pl of 6.2. Polyclonal antibodies raised against a bacterially expressed fragment of the cDNA recognise 160 kDa subunit of purified calf thymus CPSF. The sequence contains a possible nuclear localisation signal but none of the known RNA binding motifs. It does, however, show sequence similarities to a UV-damaged DNA binding protein (UVdDb).
mRNA前体的3'加工依赖于多种蛋白质因子。其中之一，切割和聚腺苷酸化特异性因子（CPSF）对于mRNA前体的切割以及尾部延伸反应都是必需的。我们已获得编码160 kDa亚基的互补DNA，此前已证明该亚基与AAUAAA聚腺苷酸化信号相互作用。这些cDNA编码一个由1444个氨基酸组成的开放阅读框。翻译后的蛋白质计算分子量为161 kDa，预测的等电点为6.2。针对cDNA的细菌表达片段产生的多克隆抗体可识别纯化的小牛胸腺CPSF的160 kDa亚基。该序列包含一个可能的核定位信号，但没有已知的RNA结合基序。然而，它确实与紫外线损伤的DNA结合蛋白（UVdDb）显示出序列相似性。
