Abendroth F D, Peterson S R, Galman M, Suwa A, Hardin J A, Dynan W S
Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309, USA.
Nucleic Acids Res. 1995 Jul 25;23(14):2770-4. doi: 10.1093/nar/23.14.2770.
We have characterized the ability of various human autoimmune sera to react with RNA polymerase II transcription factors. One serum, which strongly inhibited transcription in a cell-free system, was shown to contain antibodies directed against human TFIIB. The serum did not show reactivity against the other general transcription factors, including human TBP, TFIIE and TFIIF. The inhibition of transcription was directly attributable to depletion of TFIIB activity, as demonstrated by reconstitution of activity with recombinant TFIIB. It has long been recognized that components of the RNA processing machinery are major human autoantigens. The present results show that at least one general transcription factor required for messenger RNA synthesis is an autoantigen as well.
我们已经对多种人类自身免疫血清与RNA聚合酶II转录因子发生反应的能力进行了表征。其中一种血清在无细胞系统中强烈抑制转录，结果显示该血清含有针对人类TFIIB的抗体。该血清对其他通用转录因子，包括人类TBP、TFIIE和TFIIF，未表现出反应性。转录抑制直接归因于TFIIB活性的耗尽，重组TFIIB恢复活性证明了这一点。长期以来人们已经认识到，RNA加工机制的成分是主要的人类自身抗原。目前的结果表明，信使RNA合成所需的至少一种通用转录因子也是一种自身抗原。
