Chang H Y, Sei H, Morita Y
Department of Physiology, School of Medicine, University of Tokushima, Japan.
Physiol Behav. 1995 Jun;57(6):1019-24. doi: 10.1016/0031-9384(95)00011-7.
Vitamin B12 (VB12) has been reported to normalize the entrainment of circadian rhythms in the non-24-h sleep wake cycle and delayed sleep phase insomnia in humans. The purpose of this work was to clarify whether the peripheral administration of VB12 has any sleep-promoting effect on the sleep-wake rhythm in freely moving rats. After a baseline day of saline infusion. VB12 (500 micrograms/kg/day) was administered continuously for 4 days via the jugular vein. Polysomnographic recordings were carried out concurrently. In both the light and the 24-h periods, the amount of non-rapid eye movement (NREM) sleep increased significantly on VB12-days 2 and 3, while the amount of REM sleep increased significantly on VB12-day 2. In the light period, the increase in NREM sleep was due to increased duration of the episode, while the tendency to an increase in REM sleep was due to an increased number of episodes. Changes in the diurnal sleep-wake rhythm tended to appear in the earlier light period. The serum VB12 concentrations in the VB12 group were 40 times higher than in controls. These findings suggest that peripherally infused VB12 has promoting effects on the rat's sleep, especially in the light period.
据报道，维生素B12（VB12）可使非24小时睡眠-清醒周期中的昼夜节律以及人类的睡眠时相延迟性失眠恢复正常。这项研究的目的是阐明外周给予VB12是否对自由活动大鼠的睡眠-清醒节律有促进睡眠的作用。在生理盐水输注的基线日之后，通过颈静脉连续4天给予VB12（500微克/千克/天），同时进行多导睡眠图记录。在光照期和24小时期间，非快速眼动（NREM）睡眠量在VB12给药的第2天和第3天显著增加，而快速眼动（REM）睡眠量在VB12给药的第2天显著增加。在光照期，NREM睡眠的增加是由于单次睡眠时长增加，而REM睡眠增加的趋势是由于睡眠次数增加。昼夜睡眠-清醒节律的变化倾向于出现在更早的光照期。VB12组的血清VB12浓度比对照组高40倍。这些发现表明，外周注入VB12对大鼠的睡眠有促进作用，尤其是在光照期。
