Li W, Eidman K, Gehrz R C, Kari B
Biomedical Research Center, St. Paul Children's Hospital, St. Paul, Minnesota 55102, USA.
Virus Res. 1995 May;36(2-3):163-75. doi: 10.1016/0168-1702(94)00117-u.
The UL100 gene of human cytomegalovirus (HCMV) has been shown to encode an envelope glycoprotein that might play an important role in HCMV infection. Using the HCMV UL100 gene as a probe in low stringency hybridization studies, we were able to identify the putative UL100 homologous gene of murine cytomegalovirus (MCMV), strain Smith. The MCMV gene was sequenced and localized to the left end of the EcoRI fragment H on the MCMV physical map. This genomic location is similar to that found for the HCMV UL100 gene. The MCMV UL100 mRNA is 1.6 kb in size and is expressed exclusively in the late stages of infection. The 5' and 3' boundaries of the transcript were determined. The open reading frame (ORF) of the UL100 gene could encode a protein of 371 amino acid residues with a calculated molecular mass of 42 kDa. Computer analysis of the deduced amino acid sequence of this gene predicted the presence of eight transmembrane domains and four N-linked glycosylation sites in the protein. Sequence comparison revealed that this putative protein shares similarity with the predicted UL100 homologs of several other herpesviruses, and is most similar to the HCMV UL100 protein (47% identity).
人类巨细胞病毒（HCMV）的UL100基因已被证明可编码一种包膜糖蛋白，该蛋白可能在HCMV感染中发挥重要作用。在低严谨度杂交研究中，我们使用HCMV UL100基因作为探针，成功鉴定出了鼠巨细胞病毒（MCMV）史密斯株的假定UL100同源基因。对该MCMV基因进行了测序，并将其定位到MCMV物理图谱上EcoRI片段H的左端。这一基因组位置与HCMV UL100基因的位置相似。MCMV UL100 mRNA大小为1.6 kb，仅在感染后期表达。确定了该转录本的5'和3'边界。UL100基因的开放阅读框（ORF）可编码一个由371个氨基酸残基组成的蛋白质，计算分子量为42 kDa。对该基因推导的氨基酸序列进行计算机分析预测，该蛋白质中存在八个跨膜结构域和四个N-连接糖基化位点。序列比较显示，这种假定的蛋白质与其他几种疱疹病毒预测的UL100同源物具有相似性，并且与HCMV UL100蛋白最为相似（同一性为47%）。
