Iurov S V, Pchelintsev S Iu, Denisov L A, Afanas'eva S S, Uraeva V I, Denisova N B, Cherenkova G V, Vorob'ev A A
Zh Mikrobiol Epidemiol Immunobiol. 1995 Mar-Apr(2):64-7.
The influence of exogenic human recombinant tumor necrosis factor (TNF-alpha) on antibody production in mice immunized with the preparation of F.tularensis outer membranes (OM) was studied. TNF-alpha was injected into mice in doses of 0.001-10 I.U. before and simultaneously with the injection of the preparation of F.tularensis OM. The levels of tularemia antibodies, determined by ELISA techniques, and the number of antibody-producing cells (APC) were studied. The study revealed that recombinant TNF-alpha in the range of doses used in this investigation stimulated the formation of humoral immunity. The injection of TNF-alpha in a dose of 0.001 I.U. was found to produce the most pronounced effect on the level of tularemia antibodies and the number of antibody-producing cells. The use TNF-alpha as immunomodulator made it possible to decrease the dose of the preparation of F.tularensis OM introduced as immunogenic agent without essential changes in the number of splenic APC and in the level of tularemia antibodies.
研究了外源性人重组肿瘤坏死因子（TNF-α）对用土拉弗朗西斯菌外膜（OM）制剂免疫的小鼠抗体产生的影响。在注射土拉弗朗西斯菌OM制剂之前及同时，以0.001 - 10国际单位的剂量给小鼠注射TNF-α。采用ELISA技术测定土拉菌病抗体水平，并研究抗体产生细胞（APC）的数量。研究发现，本研究中使用的剂量范围内的重组TNF-α刺激了体液免疫的形成。发现以0.001国际单位的剂量注射TNF-α对土拉菌病抗体水平和抗体产生细胞数量产生最显著的影响。将TNF-α用作免疫调节剂能够在脾APC数量和土拉菌病抗体水平无实质性变化的情况下，降低作为免疫原性制剂引入的土拉弗朗西斯菌OM制剂的剂量。
