Iurov S V, Pchelintsev S Iu, Afanas'ev S S, Vorob'ev A A, Urakov N N, Cherenkova G V, Denisova N B, Fedortsov K K
Zh Mikrobiol Epidemiol Immunobiol. 1991 Aug(8):54-8.
Antibody formation in animals immunized with one of the components of F. tularensis surface structures was studied. The time course of antibody formation in 20 hamadryas baboons was studied in the passive hemagglutination (PHA) test, microagglutination (MA) test, and indirect enzyme immunoassay, used for the determination of IgG, IgA and IgM antibodies. The character of antibody response in the animals immunized with components of F. tularensis surface structures (S-complex) and with live tularemia vaccine was compared. The study revealed that immunization with the S-complex induced the formation of antibodies detected by all three methods. Antibody formation to the S-complex was found to be dose-dependent. With the increase of the injected dose of the S-complex, antibody titers determined in the PHA test decreased and those determined in the MA test increased, which was seemingly due to the induction of antibodies differing in their isotypes. After immunization with the S-complex the levels of IgG antibodies were lower and the levels of IgM antibodies by day 28 after immunization higher than after the injection of live tularemia vaccine.
研究了用土拉弗朗西斯菌表面结构的一种成分免疫动物后抗体的形成情况。在被动血凝试验（PHA）、微量凝集试验（MA）和间接酶免疫测定中研究了20只阿拉伯狒狒抗体形成的时间进程，这些试验用于测定IgG、IgA和IgM抗体。比较了用土拉弗朗西斯菌表面结构成分（S复合物）免疫的动物和用活兔热病疫苗免疫的动物的抗体反应特征。研究发现，用S复合物免疫可诱导通过所有三种方法检测到的抗体形成。发现针对S复合物的抗体形成呈剂量依赖性。随着S复合物注射剂量的增加，PHA试验中测定的抗体滴度降低，而MA试验中测定的抗体滴度升高，这似乎是由于诱导了不同同种型的抗体。用S复合物免疫后，IgG抗体水平低于注射活兔热病疫苗后，而免疫后第28天的IgM抗体水平高于注射活兔热病疫苗后。
