前列腺素D2和E2对体外培养的人上呼吸道纤毛纤毛摆动频率的影响。

Effects of prostaglandins D2 and E2 on ciliary beat frequency of human upper respiratory cilia in vitro.

作者信息

Schuil P J, Ten Berge M, Van Gelder J M, Graamans K, Huizing E H

机构信息

Department of Otorhinolaryngology, University Hospital Utrecht, The Netherlands.

出版信息

Acta Otolaryngol. 1995 May;115(3):438-42. doi: 10.3109/00016489509139344.

DOI:10.3109/00016489509139344

PMID:7653268

Abstract

Diminished mucociliary transport can occur in a type-I (Ig-E mediated) allergic reaction. We determined the effects of the allergy mediators prostaglandin D2 (PGD2) and prostaglandin E2 (PGE2) on the ciliary beat frequency (CBF) of human upper respiratory cilia in vitro. Human adenoid tissue was used as the source for ciliated epithelium. CBF was measured by a computerized photo-electric method. PGD2 (10(-8)-10(-5) M, n = 7) showed no statistically significant effect on CBF. PGE2 (10(-9)-10(-6) M, n = 10) caused a significant dose-dependent stimulation, with a maximum of 37% (ANOVA, p < 0.001). Thus prostaglandins D2 and E2 do not exert a direct negative influence on ciliary activity, which could account for a decrease in mucociliary transport. The stimulating effect of PGE2 may be relevant in promoting mucociliary clearance in vivo.

摘要

黏液纤毛运输功能减弱可发生在I型（Ig - E介导的）过敏反应中。我们在体外测定了过敏介质前列腺素D2（PGD2）和前列腺素E2（PGE2）对人上呼吸道纤毛的纤毛摆动频率（CBF）的影响。人腺样体组织用作纤毛上皮的来源。通过计算机化光电方法测量CBF。PGD2（10^(-8) - 10^(-5) M，n = 7）对CBF无统计学上的显著影响。PGE2（10^(-9) - 10^(-6) M，n = 10）引起显著的剂量依赖性刺激，最大刺激为37%（方差分析，p < 0.001）。因此，前列腺素D2和E2不会对纤毛活动产生直接的负面影响，这可能解释了黏液纤毛运输功能的下降。PGE2的刺激作用可能与促进体内黏液纤毛清除有关。