Rocchi P, Ferreri A M, Simone G, Magrini E, Cavallazzi L, Paolucci G
Centro Interdipartimentale di Ricerche sul Cancro Giorgio Prodi, Università di Bologna, Italy.
Anticancer Res. 1995 Jul-Aug;15(4):1381-5.
We investigated the effect on differentiation of genistein, an inhibitor of tyrosine protein kinase, and 1-(-5 isoquinolinylsulfonyl)-2-methylpiperazine (H7), an inhibitor of protein kinase C, in neuroblastoma cell lines. Growth inhibition and expression of morphological and biochemical properties were examined in the human neuroblastoma cell lines TS12 and SJNKP. Genistein and H7 induced neurite outgrowth, increased acetylcholinesterase activity and cell growth inhibition in both cell lines. These results underline that tyrosine protein kinase and protein kinase C may play a key role in the control of differentiation and proliferation of neural cells.
我们研究了酪氨酸蛋白激酶抑制剂染料木黄酮和蛋白激酶C抑制剂1-（-5异喹啉磺酰基）-2-甲基哌嗪（H7）对神经母细胞瘤细胞系分化的影响。在人神经母细胞瘤细胞系TS12和SJNKP中检测了生长抑制以及形态学和生化特性的表达。染料木黄酮和H7在这两种细胞系中均诱导了神经突生长、增加了乙酰胆碱酯酶活性并抑制了细胞生长。这些结果强调酪氨酸蛋白激酶和蛋白激酶C可能在神经细胞分化和增殖的控制中起关键作用。
