细菌脂多糖诱导小鼠腹腔巨噬细胞中的胱氨酸转运活性。
Induction of cystine transport activity in mouse peritoneal macrophages by bacterial lipopolysaccharide.
作者信息
Sato H, Fujiwara K, Sagara J, Bannai S
机构信息
Department of Biochemistry, University of Tsukuba, Ibaraki, Japan.
出版信息
Biochem J. 1995 Sep 1;310 ( Pt 2)(Pt 2):547-51. doi: 10.1042/bj3100547.
The transport of cystine has been investigated in mouse peritoneal macrophages cultured in vitro. The transport activity for cystine was very low in freshly isolated macrophages but was potently induced during culture in the presence of bacterial lipopolysaccharide (LPS) at concentrations as low as 0.1 ng/ml. The transport activity for cystine was enhanced when the cells were incubated with tumour necrosis factor-alpha (TNF-alpha), but not with interferon-gamma (IFN-gamma) or interleukin-1. IFN-gamma was rather repressive in the induction of the activity by LPS or TNF-alpha. The transport activity for cystine induced by LPS has been characterized. Cystine was transported mainly by Na(+)-independent system and the uptake of cystine was inhibited by extracellular glutamate and homocysteate, but not by aspartate, indicating that the transport of cystine in macrophages treated with LPS is mediated by System xc-. Glutathione content of the macrophages increased when they were exposed to LPS, and this increase was, at least in part, attributable to the induced activity of the cystine transport.
已对体外培养的小鼠腹腔巨噬细胞中胱氨酸的转运进行了研究。新鲜分离的巨噬细胞中胱氨酸的转运活性非常低，但在浓度低至0.1 ng/ml的细菌脂多糖（LPS）存在下培养期间，其转运活性被强烈诱导。当细胞与肿瘤坏死因子-α（TNF-α）孵育时，胱氨酸的转运活性增强，但与干扰素-γ（IFN-γ）或白细胞介素-1孵育时则不然。IFN-γ对LPS或TNF-α诱导的活性具有相当的抑制作用。已对LPS诱导的胱氨酸转运活性进行了表征。胱氨酸主要通过不依赖Na⁺的系统进行转运，细胞外谷氨酸和同型半胱氨酸可抑制胱氨酸的摄取，但天冬氨酸则无此作用，这表明LPS处理的巨噬细胞中胱氨酸的转运是由xc-系统介导的。当巨噬细胞暴露于LPS时，其谷胱甘肽含量增加，这种增加至少部分归因于诱导的胱氨酸转运活性。
Zotero 插件