Lockshin R A, Zakeri Z
Department of Biological Sciences, St. John's University, Jamaica, NY 11439, USA.
Biochem Cell Biol. 1994 Nov-Dec;72(11-12):589-96. doi: 10.1139/o94-078.
Programmed cell death in intersegmental muscles and labial glands of Manduca sexta is a type II form of active cell death, in which lysosomal destruction of cytoplasm is a prominent and early feature, and the collapse of the nucleus is late and relatively modest until the bulk of the cytoplasm has been eroded. The prominent features of this collapse include an early expansion of the lysosomal compartment and a decrease in overall protein synthesis, while a small number of mRNAs persist or are upregulated. Energy resources appear to be adequate during the early stages of degeneration, and changes in levels of second messages likewise do not seem to be sufficiently dramatic to explain the failure of the tissue. There is some suggestion that DNA may not be completely intact long before the labial gland finally collapses, suggesting that it may fail under heavy load. Otherwise, we do not yet have an explanation for the precipitous drop in overall protein synthesis.
烟草天蛾节间肌和唇腺中的程序性细胞死亡是一种II型主动细胞死亡形式，其中细胞质的溶酶体破坏是一个突出且早期的特征，而细胞核的崩溃较晚且相对轻微，直到大部分细胞质被侵蚀。这种崩溃的突出特征包括溶酶体区室的早期扩张和整体蛋白质合成的减少，而少数mRNA持续存在或上调。在退化的早期阶段，能量资源似乎充足，第二信使水平的变化同样似乎不足以显著到解释组织的衰退。有迹象表明，在唇腺最终崩溃之前很久，DNA可能就不完全完整了，这表明它可能在重负下失效。否则，我们还没有对整体蛋白质合成的急剧下降做出解释。
