Sato K, Miyahara M, Saito T, Kobayashi M
Department of Surgery I, Oita Medical University, Japan.
Eur J Cancer. 1994;30A(8):1113-7. doi: 10.1016/0959-8049(94)90468-5.
To elucidate the relationship between oncogene overexpression and malignant potential of colorectal cancer, the expression of three genes, c-myc, c-fos and c-Ki-ras was examined. Northern blot hybridisation was performed on fresh tissues excised from 35 patients with primary colorectal cancer. Overexpression of c-myc mRNA was evident in 25 of the patients (71%). Tumours with c-myc overexpression had a significantly higher frequency of lymph node metastasis than did those without (68 versus 20%, P < 0.05). The survival rate tended to be poorer (P = 0.06) in patients with c-myc overexpression (79% for 3 years) than in those without it (100%). We found no significant relationship between overexpression of the other two genes and pathological features. These findings suggest that c-myc overexpression is one parameter which can be used to determine the malignant potential of colorectal cancer.
为阐明癌基因过表达与结直肠癌恶性潜能之间的关系，检测了三种基因c-myc、c-fos和c-Ki-ras的表达。对35例原发性结直肠癌患者切除的新鲜组织进行Northern印迹杂交。25例患者（71%）中c-myc mRNA明显过表达。c-myc过表达的肿瘤发生淋巴结转移的频率显著高于未过表达的肿瘤（68%对20%，P<0.05）。c-myc过表达的患者生存率往往较低（P=0.06）（3年生存率为79%），而未过表达的患者为100%。我们发现另外两种基因的过表达与病理特征之间无显著关系。这些发现表明，c-myc过表达是可用于确定结直肠癌恶性潜能的一个参数。
