Tucci M A, Benghuzzi H A
Department of Health Sciences, University of Mississippi Medical Center, Jackson 39216, USA.
Biomed Sci Instrum. 1995;31:135-40.
The use of biomaterials in biological system was extensively conducted in an in vivo environments. This method limits the evaluation and imposes an obstacle for quantitative analysis of several parameters. The development of tissue culture techniques to evaluate the bioactivity of potential organic compounds alleviated these problems and provided a reliable prediction regarding the biocompatibility of biomaterials when compared with parallel animal testing. The objective of this study is to investigate the effect of various biomedical polymers on the adhesion rate of transformed HeLa cells as a model. The HeLa cells used in this study seeded by following our standard laboratory procedure. A total of 1.5 x 10(5) cells were plated in each of the pretreated wells with various concentrations of (0.01 0.1 and 1% wt/vol) polyvaline (P-Val), polyalanine (P-Ala), polytryptophan (P-Trp), polyasparagine (P-Asn), polyaspartate (P-Asp), poly glycine (P-Gly), and buffered control. At the end of 1,4, and 24 hours the cell viability was determined by hexoamindase activity. The data obtained from this study suggest that (I) the ease of adhesion of HeLa cells were in the following order: P-Val = P-Ala > P-Gly = P-Trp = P-Asp = P-Asn > Control, (II) the rate of HeLa cells spreading was strongly influenced by both incubation time and the polymer concentration, and (III) the surface attachment of HeLa to the polymer were demonstrated to vary depending on their chemical structure and level of microporosity. Thus, overall observation led us to conclude that the surface reactivity of polymer materials be always taken into account in discussing their biocompatibility in vivo.
生物材料在生物系统中的应用在体内环境中广泛开展。这种方法限制了评估，并给几个参数的定量分析带来了障碍。与平行的动物试验相比，用于评估潜在有机化合物生物活性的组织培养技术的发展缓解了这些问题，并提供了关于生物材料生物相容性的可靠预测。本研究的目的是以转化的HeLa细胞为模型，研究各种生物医学聚合物对其黏附率的影响。本研究中使用的HeLa细胞按照我们标准的实验室程序进行接种。将总共1.5×10⁵个细胞接种到每个经过预处理的孔中，这些孔中含有不同浓度（0.01%、0.1%和1%重量/体积）的聚缬氨酸（P-Val）、聚丙氨酸（P-Ala）、聚色氨酸（P-Trp）、聚天冬酰胺（P-Asn）、聚天冬氨酸（P-Asp）、聚甘氨酸（P-Gly）以及缓冲对照。在1小时、4小时和24小时结束时，通过己糖胺酶活性测定细胞活力。从本研究中获得的数据表明：（I）HeLa细胞的黏附难易程度顺序如下：P-Val = P-Ala > P-Gly = P-Trp = P-Asp = P-Asn > 对照；（II）HeLa细胞的铺展速率受到孵育时间和聚合物浓度的强烈影响；（III）HeLa细胞与聚合物的表面附着情况被证明会因其化学结构和微孔率水平而有所不同。因此，总体观察使我们得出结论，在讨论聚合物材料在体内的生物相容性时，始终要考虑其表面反应性。
