Benghuzzi H, Puckett A, Tucci M, Roberts B
University of Mississippi Medical Center 2500 North State Street, Jackson, MS 39216, USA.
Biomed Sci Instrum. 1999;35:321-6.
The specific objectives of this study was: (I) to investigate the effect of various biomedical ceramics such as tricalcium phosphate (TCP), hydroxyapatite (HA), and aluminum-calcium-phosphorous oxide (ALCAP) on the adherence and viability of mice osteoclasts (OT) in vitro, and (II) to evaluate the role of OT that might have on the surfaces of such bioceramics. The OT cells were isolated from adult male mice and seeded at a density of 5 x 10(5) cells/well according to standard laboratory procedures. Cells were plated in each micrometer-well pretreated with ceramic capsules (HA, TCP and ALCAP) and buffered control. At the end of 1, 2, 3 and 5 days, the viability and cell number of OT were determined using an established assay. Cell number was determined in control wells with known number of cells, and a standard curve was generated by plotting absorbency units versus cell number. Biochemical analysis was performed on the aliquots obtained from the experimental and control wells at the end of each phase of the investigation. The data from this experiment suggest that: (I) OT are capable of adhering to the surface of HA, TCP and ALCAP in an in vitro environment for over a 5-day period. (II) Long-term incubation of ceramic capsules with OT revealed that the cells experienced gradual disassociation phenomenon with a greater numbers of cell detachments seen in the ALCAP contained wells. (III) SEM analysis of representative capsules demonstrated that there was an increase in the number of micro/macropores on the surface of the materials after contacting a cellular environment. This observation suggest that the material surface has been modified (TCP > HA = ALCAP). Information obtained from this study provided new insights on the interrelationship between bioceramics and the possible OT response during chronic inflammation at the site of implantation.
（I）研究各种生物医学陶瓷，如磷酸三钙（TCP）、羟基磷灰石（HA）和铝钙磷氧化物（ALCAP）对小鼠破骨细胞（OT）体外黏附和活力的影响，以及（II）评估OT在这类生物陶瓷表面可能发挥的作用。按照标准实验室程序，从成年雄性小鼠中分离出OT细胞，并以5×10⁵个细胞/孔的密度接种。将细胞接种于预先用陶瓷胶囊（HA、TCP和ALCAP）和缓冲对照预处理的每个微孔板中。在第1、2、3和5天结束时，使用既定的检测方法测定OT的活力和细胞数量。在已知细胞数量的对照孔中测定细胞数量，并通过绘制吸光度单位与细胞数量的关系生成标准曲线。在研究的每个阶段结束时，对从实验孔和对照孔中获得的等分试样进行生化分析。该实验的数据表明：（I）在体外环境中，OT能够在5天以上的时间内黏附于HA、TCP和ALCAP的表面。（II）陶瓷胶囊与OT的长期孵育表明，细胞出现逐渐解离现象，在含有ALCAP的孔中观察到更多的细胞脱落。（III）对代表性胶囊的扫描电子显微镜分析表明，在接触细胞环境后，材料表面的微孔/大孔数量增加。这一观察结果表明材料表面已被修饰（TCP>HA = ALCAP）。从本研究中获得的信息为生物陶瓷与植入部位慢性炎症期间可能的OT反应之间的相互关系提供了新的见解。
