Attawia M A, Uhrich K E, Botchwey E, Langer R, Laurencin C T
The Helen Moorehead-Laurencin Laboratory for Biomaterials Research, Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, USA.
J Orthop Res. 1996 May;14(3):445-54. doi: 10.1002/jor.1100140315.
Poly(anhydride-co-imides) are currently under study for applications involving bone. The cytotoxicity of a series of poly(anhydride-co-imides) with osteoblast-like cells (MC3T3-E1) was evaluated. The imide component of the copolymers was based on pyromellitylimidoalanine and the anhydride component was based on either sebacic acid or 1,6-bis(carboxyphenoxy)hexane. Cell adhesion and proliferation on the surfaces of the polymer discs were observed by environmental scanning electron microscopy. During the first 24 hours of attachment, the cells showed normal morphology when cultured on copolymers containing 1,6-bis(carboxyphenoxy)hexane. The cells did not adhere to the polymers containing sebacic acid, probably due to the rapid degradation of the polymer surfaces. Concurrently, the effects of polymer breakdown products on osteoblast-like cells were evaluated by studying their proliferation (cell numbers), viability (dye exclusion), morphology (light microscopy), and phenotypic expression. The morphology of osteoblast-like cells cultured in the presence of the polymer breakdown products pyromellitylimidoalanine and pyromellitic acid was found to be similar to that of the same cells grown on tissue culture polystyrene and consisted of a characteristic polygonal shape. With use of a monoclonal antibody to osteocalcin, these cells were shown to demonstrate preserved osteoblast phenotype with growth over a 21-day period. In addition, the cells reached confluency after 3-4 days, similar to cells grown on tissue culture polystyrene. This in vitro evaluation showed that the poly(anhydride-co-imides) evaluated are non-cytotoxic and may be viable biomaterials for orthopaedic applications.
聚（酸酐 - 共 - 酰亚胺）目前正在针对涉及骨骼的应用进行研究。评估了一系列聚（酸酐 - 共 - 酰亚胺）对成骨样细胞（MC3T3 - E1）的细胞毒性。共聚物的酰亚胺成分基于均苯四甲酰亚胺丙氨酸，酸酐成分基于癸二酸或1,6 - 双（羧基苯氧基）己烷。通过环境扫描电子显微镜观察聚合物圆盘表面上的细胞粘附和增殖情况。在附着的最初24小时内，当在含有1,6 - 双（羧基苯氧基）己烷的共聚物上培养时，细胞呈现正常形态。细胞未粘附于含有癸二酸的聚合物，这可能是由于聚合物表面的快速降解所致。同时，通过研究聚合物分解产物对成骨样细胞的增殖（细胞数量）、活力（染料排斥）、形态（光学显微镜）和表型表达的影响，评估了聚合物分解产物对成骨样细胞的作用。发现在聚合物分解产物均苯四甲酰亚胺丙氨酸和均苯四甲酸存在下培养的成骨样细胞的形态与在组织培养聚苯乙烯上生长的相同细胞的形态相似，呈特征性的多边形形状。使用抗骨钙素单克隆抗体显示，这些细胞在21天的生长过程中表现出保留的成骨细胞表型。此外，这些细胞在3 - 4天后达到汇合，类似于在组织培养聚苯乙烯上生长的细胞。这种体外评估表明，所评估的聚（酸酐 - 共 - 酰亚胺）无细胞毒性，可能是用于骨科应用的可行生物材料。
