Benoiton N L, Lee Y C, Steinauer R
University of Ottawa, ON, Canada.
Pept Res. 1995 Mar-Apr;8(2):108-12.
Oxazolones and symmetrical anhydrides react efficiently with amino acids if the aqueous solvent includes a high proportion of dimethylformamide, the temperature is not lowered and sodium carbonate is used for neutralizing the amino acids. Subsequent separation of the diastereomeric products by high-performance liquid chromatography on a C-18 column provides a simple method for determining enantiomers. The symmetrical anhydrides of N-ethoxycarbonylvaline and phenyl-alanine are reagents suitable for determining enantiomers. Enantiomers of arginine and histidine in a mixture of amino acids can be determined if the products of the reaction are first fractionated by a simple extraction.
如果水性溶剂中含有高比例的二甲基甲酰胺，温度不降低且使用碳酸钠中和氨基酸，恶唑酮和对称酸酐能与氨基酸有效反应。随后通过在C-18柱上进行高效液相色谱法分离非对映体产物，提供了一种测定对映体的简单方法。N-乙氧羰基缬氨酸和苯丙氨酸的对称酸酐是适用于测定对映体的试剂。如果首先通过简单萃取将反应产物分级分离，则可以测定氨基酸混合物中精氨酸和组氨酸的对映体。
