Deng J M, Behringer R R
Department of Molecular Genetics, University of Texas, M.D. Anderson Cancer Center, Houston 77030, USA.
Transgenic Res. 1995 Jul;4(4):264-9. doi: 10.1007/BF01969120.
The gene that encodes the general transcription factor known as BTF3 was disrupted in mouse embryonic stem cells in a random mutagenesis screen for developmental genes with the ROSA beta-geo (Friedrich and Soriano, 1991) retroviral gene trap vector. The BTF3 mutation was transmitted through the germline of chimaeric mice. While the endogenous BTF3 gene is ubiquitously expressed, the expression pattern of the beta-galactosidase reporter gene present in the gene trap vector in BTF3 heterozygotes was restricted. Mice homozygous for the mutant allele died soon after implantation, around embryonic day 6.5. Thus, BTF3 is essential for postimplantation development. The isolation of the BTF3 sequences in this ROSA beta-geo insertion was facilitated by a relatively simple single lacZ primer reverse transcription PCR strategy.
在一项利用ROSA β-geo（Friedrich和Soriano，1991）逆转录病毒基因捕获载体对发育基因进行的随机诱变筛选中，编码通用转录因子BTF3的基因在小鼠胚胎干细胞中被破坏。BTF3突变通过嵌合小鼠的种系传递。虽然内源性BTF3基因在全身广泛表达，但基因捕获载体中存在的β-半乳糖苷酶报告基因在BTF3杂合子中的表达模式受到限制。突变等位基因纯合的小鼠在植入后不久，即胚胎第6.5天左右死亡。因此，BTF3对植入后发育至关重要。通过一种相对简单的单lacZ引物逆转录PCR策略，有助于在该ROSA β-geo插入中分离BTF3序列。
