Potashkin J, Wentz-Hunter K, Callaci J
Department of Pharmacology and Molecular Biology, Finch University of Health Sciences/The Chicago Medical School, IL 60064, USA.
Biochim Biophys Acta. 1996 Sep 11;1308(3):182-4. doi: 10.1016/0167-4781(96)00114-5.
BTF3 is a protein initially identified in HeLa cells that may be involved in the initiation of transcription. Although its specific role in transcription is unclear, BTF3 can form a stable complex with RNA polymerase II. Recently, BTF3 has also been shown to bind to nascent polypeptide chains. We have cloned a homolog of BTF3 from the fission yeast, Schizosaccharomyces pombe. This homolog, spBTF3, encodes a putative 151 amino acid protein that shares 72% similarity with human BTF3, 73% similarity with the Caenorhabditis elegans homolog and between 52 and 53% similarity with the Saccharomyces cerevisiae homologs, EGD1 and BTT1.
BTF3是一种最初在HeLa细胞中发现的蛋白质，可能参与转录起始过程。尽管其在转录中的具体作用尚不清楚，但BTF3可与RNA聚合酶II形成稳定复合物。最近，研究还表明BTF3能与新生多肽链结合。我们从裂殖酵母粟酒裂殖酵母中克隆出了BTF3的一个同源物。这个同源物spBTF3编码一个推定的151个氨基酸的蛋白质，与人BTF3有72%的相似性，与秀丽隐杆线虫的同源物有73%的相似性，与酿酒酵母的同源物EGD1和BTT1有52%至53%的相似性。
