Chen S, Claeys M
Department of Chemistry, University of Warwick, Coventry, UK.
J Chromatogr B Biomed Appl. 1995 Apr 7;666(1):178-82. doi: 10.1016/0378-4347(94)00557-l.
An approach to the large-scale preparation of highly pure phosphatidylserine from bovine brain is described in this paper. The method is based on (i) the separation of phosphatidylserine from phosphatidylinositol in bovine brain extract by preparative aminopropyl normal-phase high-performance liquid chromatography using methanol-1 M phosphoric acid (90:10, v/v) as mobile phase and (ii) further purification of phosphatidylserine by anion-exchange chromatography. The main advantage of this approach is that polyunsaturated acid-containing molecular species of brain phosphatidylserine are not lost in the preparation procedure.
本文描述了一种从牛脑中大规模制备高纯度磷脂酰丝氨酸的方法。该方法基于：（i）使用甲醇 - 1M磷酸（90:10，v/v）作为流动相，通过制备型氨丙基正相高效液相色谱法从牛脑提取物中分离磷脂酰丝氨酸和磷脂酰肌醇；（ii）通过阴离子交换色谱法进一步纯化磷脂酰丝氨酸。这种方法的主要优点是脑磷脂酰丝氨酸中含多不饱和酸的分子种类在制备过程中不会损失。
