Edwards A M, Darst S A, Hemming S A, Li Y, Kornberg R D
Department of Cell Biology, Stanford University School of Medicine, California 94305, USA.
Nat Struct Biol. 1994 Mar;1(3):195-7. doi: 10.1038/nsb0394-195.
The growth of three-dimensional protein crystals is seeded by two-dimensional crystals formed on lipid layers. Such crystallization occurs faster and at lower precipitant and protein concentrations than conventional crystal growth. This approach may also allow the crystallization of proteins that resist attempts at crystal growth by other means.
三维蛋白质晶体的生长是由在脂质层上形成的二维晶体引发的。与传统晶体生长相比,这种结晶过程发生得更快,所需沉淀剂和蛋白质浓度更低。这种方法还可能使那些用其他方法难以结晶的蛋白质得以结晶。