Wastie S, Buttery P J, Vernon R G
University of Nottingham, Department of Applied Biochemistry and Food Science, Loughborough, U.K.
Comp Biochem Physiol C Pharmacol Toxicol Endocrinol. 1995 May;111(1):13-8. doi: 10.1016/0742-8413(95)00032-3.
Mechanisms by which the glucocorticoid analogue dexamethasone and growth hormone modulate insulin action in sheep adipose tissue have been investigated. Maintenance of sheep adipose tissue in culture for 48 hr in the absence of exogenous hormones resulted in a decrease in insulin binding to adipocyte membranes; this was prevented by the inclusion of 10 nM dexamethasone during culture. Tissue culture for 48 hr with insulin itself decreased insulin binding to adipocyte membranes, whereas tissue culture with growth hormone had no effect on insulin binding. Neither dexamethasone nor growth hormone altered the ability of insulin to decrease insulin binding to its receptor. The study shows that the insulin-antagonistic effects of growth hormone on sheep adipose tissue metabolism are due to an effect subsequent to insulin binding to its receptor, whereas some of the effects of dexamethasone may be due to an increase in the insulin-binding capacity of membranes.
糖皮质激素类似物地塞米松和生长激素调节绵羊脂肪组织中胰岛素作用的机制已得到研究。在无外源激素的情况下将绵羊脂肪组织培养48小时会导致胰岛素与脂肪细胞膜结合减少；培养过程中加入10 nM地塞米松可防止这种情况发生。用胰岛素本身进行48小时的组织培养会降低胰岛素与脂肪细胞膜的结合，而用生长激素进行组织培养对胰岛素结合没有影响。地塞米松和生长激素均未改变胰岛素降低其与受体结合的能力。该研究表明，生长激素对绵羊脂肪组织代谢的胰岛素拮抗作用是由于胰岛素与其受体结合后的效应，而地塞米松的一些效应可能是由于膜的胰岛素结合能力增加所致。
