Beattie J, Vernon R G
Hannah Research Institute, Scotland, UK.
Mol Cell Biochem. 1995 Apr 26;145(2):151-7. doi: 10.1007/BF00935487.
Using a solution phase assay we have demonstrated that sheep adipose tissue explants secrete insulin-like growth factor binding proteins (IGFBPs) when cultured in serum-free medium over a 24 h period. Further, we demonstrate that secretion of IGFBP(s) is inhibited (up to 50%) by incubation of the cultures in the presence of 10(-8) M dexamethasone. This inhibitory effect is overcome when insulin (10 ng/ml) and ovine growth hormone (100 ng/ml) are incubated together (but not separately) with glucocorticoid. Further characterisation of this IGF binding activity by high performance size exclusion chromatography and Western ligand blot analysis indicated that under our culture conditions sheep adipose tissue explants secrete one predominant 21 kDa IGFBP and it is this BP which is hormonally regulated as described above. We discuss our results in the context of endocrine/paracrine/autocrine control of adipose tissue metabolism and differentiation.
使用一种溶液相分析法，我们已经证明，绵羊脂肪组织外植体在无血清培养基中培养24小时时会分泌胰岛素样生长因子结合蛋白（IGFBPs）。此外，我们证明，在10^(-8) M地塞米松存在的情况下培养这些外植体，IGFBP(s)的分泌会受到抑制（高达50%）。当胰岛素（10 ng/ml）和绵羊生长激素（100 ng/ml）与糖皮质激素一起（但不是分别）孵育时，这种抑制作用会被克服。通过高效尺寸排阻色谱法和Western配体印迹分析对这种IGF结合活性进行的进一步表征表明，在我们的培养条件下，绵羊脂肪组织外植体分泌一种主要的21 kDa IGFBP，正是这种结合蛋白受到上述激素的调节。我们在脂肪组织代谢和分化的内分泌/旁分泌/自分泌控制的背景下讨论我们的结果。
