Guo D, Horio D T, Grove J S, Dashwood R H
Department of Environmental Biochemistry, University of Hawaii, Honolulu 96822, USA.
Cancer Lett. 1995 Aug 16;95(1-2):161-5. doi: 10.1016/0304-3835(95)03882-w.
This report describes a 1-year carcinogenicity bioassay in which male F344 rats received 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) by gavage 2-3 times per week throughout the first 35 weeks of study, while 0.1% sodium/copper chlorophyllin (CHL) was given concomitantly in the drinking water. The carcinogen dose was calculated to provide an average exposure of 4.2 mg IQ/rat per day, equivalent to that reported in previous carcinogenicity study in which rats were given 0.03% IQ in the diet. Adjusted for length of follow-up, inhibition was highly significant for total tumor burden (P < 0.001), and for specific sites was significant in the small intestine and Zymbal's gland (P < or = 0.1), and in the liver (P < 0.01). In rats given CHL, the first onset of tumors in the Zymbal's gland was delayed from week 20 to 36, but was shortened from week 31 to 26 in the skin (P < 0.05). These results support a chemopreventive role for CHL in several of the major target organs for IQ tumorigenesis in the rat, but raise concerns over possible deleterious effects in the skin.
本报告描述了一项为期1年的致癌性生物测定，其中雄性F344大鼠在研究的前35周每周经口灌胃给予2-氨基-3-甲基咪唑[4,5-f]喹啉（IQ）2至3次，同时在饮用水中给予0.1%的叶绿素铜钠盐（CHL）。计算致癌物剂量以提供平均每日暴露量为4.2 mg IQ/大鼠，这与之前在饮食中给予大鼠0.03% IQ的致癌性研究中报告的剂量相当。经随访时间调整后，总肿瘤负荷的抑制作用非常显著（P < 0.001），在小肠和齐默尔氏腺（P ≤ 0.1）以及肝脏（P < 0.01）等特定部位的抑制作用也很显著。在给予CHL的大鼠中，齐默尔氏腺肿瘤的首次出现从第20周推迟到第36周，但皮肤肿瘤的首次出现从第31周缩短到第26周（P < 0.05）。这些结果支持CHL在大鼠IQ致癌作用的几个主要靶器官中具有化学预防作用，但也引发了对皮肤可能产生有害影响的担忧。
