直接作用的肝癌致癌物N-亚硝基-2-乙酰氨基芴和N-乙酰氧基-2-乙酰氨基芴诱导人p53基因组DNA外显子7-8处出现的优先促突变损伤。

Preferential promutagenic lesions at exons 7-8 of human p53 genomic DNA induced by the direct-acting hepatocarcinogens N-nitroso-2-acetylaminofluorene and N-acetoxy-2-acetylaminofluorene.

作者信息

Ho Y S, Lin J K

机构信息

Institute of Biochemistry, College of Medicine, National Taiwan University, Taipei, Republic of China.

出版信息

Cancer Lett. 1995 Aug 16;95(1-2):39-47. doi: 10.1016/0304-3835(95)03863-r.

DOI:10.1016/0304-3835(95)03863-r

PMID:7656242

Abstract

DNA polymerase fingerprint analysis (DPFA) was employed for identifying DNA-carcinogen adduct formation in the human p53 and lac gene sequence. Two 'hot regions' at codons 223-250 and 257-283 of the p53 gene were easily attacked by nitroso-2-acetylaminofluorene or acetoxy-2-acetylaminofluorene. However, the promutational lesions in lac gene were rather randomly distributed. The chemical treated plasmid (pUC 19) which contains lac gene were transfected into Escherichia coli JM109 cells and the induced lac gene mutants were selected with X-Gal plate as indicated by the appearance of white colonies. No mutational hot regions were found in the lac gene.

摘要

采用DNA聚合酶指纹分析（DPFA）来鉴定人p53和lac基因序列中DNA - 致癌物加合物的形成。p53基因密码子223 - 250和257 - 283处的两个“热点区域”很容易受到亚硝基 - 2 - 乙酰氨基芴或乙酰氧基 - 2 - 乙酰氨基芴的攻击。然而，lac基因中的促突变损伤分布较为随机。将含有lac基因的化学处理质粒（pUC 19）转染到大肠杆菌JM109细胞中，并如白色菌落的出现所示，用X - Gal平板筛选诱导的lac基因突变体。在lac基因中未发现突变热点区域。