紫外线照射或N-乙酰氧基-N-2-乙酰氨基芴修饰的质粒DNA的修复与诱变
Repair and mutagenesis of plasmid DNA modified by ultraviolet irradiation or N-acetoxy-N-2-acetylaminofluorene.
作者信息
Schmid S E, Daune M P, Fuchs R P
出版信息
Proc Natl Acad Sci U S A. 1982 Jul;79(13):4133-7. doi: 10.1073/pnas.79.13.4133.
Abstract
Plasmid DNA was modified in vitro to various extents with N-acetoxy-N-2-acetylaminofluorene or UV irradiation. The modified plasmid DNAs were then used to transform Escherichia coli strains having different repair capabilities. Both survival and mutagenesis frequencies of the plasmid were measured as a function of the number of lesions per plasmid molecule. The majority of N-2-acetylaminofluorene (AAF) adducts, like thymine dimers, were repaired by the excision (uvrA+-dependent) pathway. In rec+ strains, dose-dependent mutagenesis occurred in either AAF- or UV-modified plasmid DNA. This is in contrast with results obtained in recA- strains, in which only AAF adducts gave rise to a lower, but dose-dependent, mutagenesis frequency. In these recA- strains there was no UV mutagenesis. Unlike what is observed with phages, induction of the "SOS" functions by UV irradiation of the bacteria prior to transformation did not increase the survival or the mutagenesis of the plasmid.
摘要
用N-乙酰氧基-N-2-乙酰氨基芴或紫外线照射对质粒DNA进行不同程度的体外修饰。然后用修饰后的质粒DNA转化具有不同修复能力的大肠杆菌菌株。测定质粒的存活率和诱变频率与每个质粒分子损伤数的函数关系。大多数N-2-乙酰氨基芴（AAF）加合物，如胸腺嘧啶二聚体，通过切除（依赖uvrA+）途径进行修复。在rec+菌株中，AAF或紫外线修饰的质粒DNA中均发生剂量依赖性诱变。这与recA-菌株中获得的结果相反，在recA-菌株中，只有AAF加合物产生较低但剂量依赖性的诱变频率。在这些recA-菌株中没有紫外线诱变。与噬菌体不同，在转化前对细菌进行紫外线照射诱导“SOS”功能并不会增加质粒的存活率或诱变率。
相似文献
1
Repair and mutagenesis of plasmid DNA modified by ultraviolet irradiation or N-acetoxy-N-2-acetylaminofluorene.
Proc Natl Acad Sci U S A. 1982 Jul;79(13):4133-7. doi: 10.1073/pnas.79.13.4133.
2
Carcinogen-induced mutation spectrum in wild-type, uvrA and umuC strains of Escherichia coli. Strain specificity and mutation-prone sequences.
J Mol Biol. 1984 Jul 25;177(1):33-51. doi: 10.1016/0022-2836(84)90056-1.
3
Specificity of N-acetoxy-N-2-acetylaminofluorene-induced frameshift mutation spectrum in mismatch repair deficient Escherichia coli strains mutH, L, S and U.
J Mol Biol. 1986 Aug 5;190(3):499-507. doi: 10.1016/0022-2836(86)90018-5.
4
Stimulation of recombination between homologous sequences on plasmid DNA and chromosomal DNA in Escherichia coli by N-acetoxy-2-acetylaminofluorene.
Proc Natl Acad Sci U S A. 1984 May;81(9):2831-5. doi: 10.1073/pnas.81.9.2831.
5
pBR322 plasmid DNA modified with 2-acetylaminofluorene derivatives: transforming activity and in vitro strand cleavage by the Escherichia coli uvrABC endonuclease.
EMBO J. 1984 Apr;3(4):757-60. doi: 10.1002/j.1460-2075.1984.tb01880.x.
6
Direct comparison, in humans resting lymphocytes, of the inter-individual variations in unscheduled DNA synthesis induced by N-acetoxy-2-acetylaminofluorene and ultraviolet irradiation.
Mutat Res. 1980 Dec;73(2):349-61. doi: 10.1016/0027-5107(80)90200-6.
7
Mutational and recombinational events in carcinogen-modified plasmid DNA. Influence of host-cell repair genes.
Mutat Res. 1985 Jan-Mar;145(1-2):25-34. doi: 10.1016/0167-8817(85)90036-7.
8
Genetic control of AAF-induced mutagenesis at alternating GC sequences: an additional role for RecA.
Mol Gen Genet. 1989 Jan;215(2):306-11. doi: 10.1007/BF00339733.
9
Different levels of induction of RecA protein in E. coli (PQ 10) after treatment with two related carcinogens.
Nucleic Acids Res. 1983 Aug 11;11(15):5235-42. doi: 10.1093/nar/11.15.5235.
10
Biochemical analysis of UV mutagenesis in Escherichia coli by using a cell-free reaction coupled to a bioassay: identification of a DNA repair-dependent, replication-independent pathway.
Proc Natl Acad Sci U S A. 1992 Apr 15;89(8):3300-4. doi: 10.1073/pnas.89.8.3300.
引用本文的文献
1
Recombination Mediator Proteins: Misnomers That Are Key to Understanding the Genomic Instabilities in Cancer.
Genes (Basel). 2022 Feb 27;13(3):437. doi: 10.3390/genes13030437.
2
Rho-dependent transcription termination in bacteria recycles RNA polymerases stalled at DNA lesions.
Nat Commun. 2019 Mar 14;10(1):1207. doi: 10.1038/s41467-019-09146-5.
3
Inefficient replication reduces RecA-mediated repair of UV-damaged plasmids introduced into competent Escherichia coli.
Plasmid. 2012 Sep;68(2):113-24. doi: 10.1016/j.plasmid.2012.04.002. Epub 2012 Apr 19.
4
Cellular strategies for accommodating replication-hindering adducts in DNA: control by the SOS response in Escherichia coli.
Proc Natl Acad Sci U S A. 1996 Jul 23;93(15):7805-10. doi: 10.1073/pnas.93.15.7805.
5
Temporal distinction between repair and mutagenesis of benzopyrene adducts after SOS induction in Escherichia coli.
J Bacteriol. 1983 Nov;156(2):926-30. doi: 10.1128/jb.156.2.926-930.1983.
6
pBR322 plasmid DNA modified with 2-acetylaminofluorene derivatives: transforming activity and in vitro strand cleavage by the Escherichia coli uvrABC endonuclease.
EMBO J. 1984 Apr;3(4):757-60. doi: 10.1002/j.1460-2075.1984.tb01880.x.
7
Stimulation of recombination between homologous sequences on plasmid DNA and chromosomal DNA in Escherichia coli by N-acetoxy-2-acetylaminofluorene.
Proc Natl Acad Sci U S A. 1984 May;81(9):2831-5. doi: 10.1073/pnas.81.9.2831.
8
Directed mutagenesis method for analysis of mutagen specificity: application to ultraviolet-induced mutagenesis.
Proc Natl Acad Sci U S A. 1983 Jan;80(1):237-41. doi: 10.1073/pnas.80.1.237.
9
Rearrangement and mutagenesis of a shuttle vector plasmid after passage in mammalian cells.
Proc Natl Acad Sci U S A. 1983 May;80(10):3010-4. doi: 10.1073/pnas.80.10.3010.
10
Error-prone mutagenesis detected in mammalian cells by a shuttle vector containing the supF gene of Escherichia coli.
Mol Cell Biol. 1984 Oct;4(10):2227-30. doi: 10.1128/mcb.4.10.2227-2230.1984.
本文引用的文献
1
Induction of Mutations in a Bacterial Virus.
Proc Natl Acad Sci U S A. 1953 Jul;39(7):628-36. doi: 10.1073/pnas.39.7.628.
2
[INFLUENCE OF BACTERIAL HOST GENOTYPE ON LAMBDA PHAGE MUTATION PRODUCED BY ULTRAVIOLET RADIATION].
C R Hebd Seances Acad Sci. 1965 Feb 1;260:1510-3.
3
The interaction of phage S13 with ultraviolet-irradiated host cells and properties of the ultraviolet-irradiated phage.
Virology. 1960 Nov;12:431-49. doi: 10.1016/0042-6822(60)90165-3.
4
[Mutation of bacteriophage induced by irradiation of bacterial host alone before infection].
C R Hebd Seances Acad Sci. 1954 Feb 8;238(6):732-4.
5
A transversion mutation hypothesis for chemical carcinogenesis by N2-substitution of guanine in DNA.
Chem Biol Interact. 1980 Sep;31(3):255-63. doi: 10.1016/0009-2797(80)90014-9.
6
Hot spots of frameshift mutations induced by the ultimate carcinogen N-acetoxy-N-2-acetylaminofluorene.
Nature. 1981 Dec 17;294(5842):657-9. doi: 10.1038/294657a0.
7
Radiation-induced base substitution mutagenesis in single-stranded DNA phage M13.
Nature. 1981 Nov 12;294(5837):180-2. doi: 10.1038/294180a0.
8
Efficiency of Escherichia coli repair processes on uv-damaged transforming plasmid DNA.
Plasmid. 1981 Mar;5(2):213-20. doi: 10.1016/0147-619x(81)90022-6.
9
Differential mutagenicities of 6 N-nitroso-N-alkylurea derivatives in Escherichia coli strains with different DNA-repair capacities.
Mutat Res. 1980 Dec;79(4):319-25. doi: 10.1016/0165-1218(80)90155-x.
10
Genetic factors in Escherichia coli that affect cell killing and mutagenesis induced by benzo(a)pyrene-7,8-dihydrodiol 9,10-oxide.
Cancer Res. 1980 Oct;40(10):3508-11.
Zotero 插件