Zhu W, Zheng J, Fang W
Department of Pathology, Beijing Medical University.
Zhonghua Bing Li Xue Za Zhi. 1995 Jun;24(3):136-8.
Single cell clones were isolated from metastatic human lung giant cell carcinoma (PG) using limited dilution method. After preliminary screening by in vitro invasion assay, tumorigenicity and spontaneous metastasis assay in nude mice, three cloned PG cell sublines (PGBE1, PGCL3 and PGLH7) were selected for further observation on morphology, chromosome number, in vitro growth and invasion assays, tumorigenicity and spontaneous metastasis to lung and lymph nodes in nude mice. We found that there was some difference on spontaneous metastasis to lung and lymph nodes in nude mice and in vitro invasive abilities between PGBE1 and PGLH7. But there were no obvious differences in other biological features among the three sublines. The results show that the PG cell line consists of different subpopulations of cells with different metastatic properties. These sublines may be valuable for further study on the molecular mechanisms of cancer metastasis.
采用有限稀释法从转移性人肺巨细胞癌（PG）中分离出单细胞克隆。经体外侵袭试验、裸鼠致瘤性和自发转移试验初步筛选后，选取三个克隆的PG细胞亚系（PGBE1、PGCL3和PGLH7），进一步观察其形态、染色体数目、体外生长和侵袭试验、致瘤性以及在裸鼠体内向肺和淋巴结的自发转移情况。我们发现，PGBE1和PGLH7在裸鼠体内向肺和淋巴结的自发转移以及体外侵袭能力方面存在一些差异。但这三个亚系在其他生物学特性上没有明显差异。结果表明，PG细胞系由具有不同转移特性的不同细胞亚群组成。这些亚系可能对进一步研究癌症转移的分子机制具有重要价值。
