Zhu W Y, Zheng J, Fang W G
Department of Pathology, Beijing Medical University.
Zhonghua Bing Li Xue Za Zhi. 1994 Dec;23(6):347-50.
Pre-treatment of metastatic human lung cancer cell subline (PGCL3) with all trans retinoic acid (RA) resulted in inhibition of cell growth in vitro and invasion through the reconstituted basement membrane. RA was also noticed to inhibit the experimental metastatic ability of PGCL3. Data showed that 5/6, 3/6 and 2/6 of the nude mice developed lung colonization in the control, the 5 mumol/L and the 10 mumol/L RA treated PGCL3 cells respectively. Data from DNA-RNA dot blot hybridization further showed that the 10 mumol/L RA treated cells expressed high levels of the human tissue inhibitors of metalloproteinases (Timp-1 and Timp-2) in comparing to the untreated cells. These results may help to clarify the mechanism of RA-induced inhibition effect on tumor invasion and metastasis.
用全反式维甲酸（RA）对转移性人肺癌细胞亚系（PGCL3）进行预处理，可导致体外细胞生长受到抑制，并抑制其穿过重组基底膜的侵袭能力。还发现RA可抑制PGCL3的实验性转移能力。数据显示，在对照组、5 μmol/L和10 μmol/L RA处理的PGCL3细胞组中，分别有5/6、3/6和2/6的裸鼠出现肺部定植。DNA-RNA斑点杂交数据进一步显示，与未处理的细胞相比，10 μmol/L RA处理的细胞中人类金属蛋白酶组织抑制剂（Timp-1和Timp-2）表达水平较高。这些结果可能有助于阐明RA诱导的对肿瘤侵袭和转移的抑制作用机制。
