Ke C S, Li W H
Department of Pathology, Chinese PLA General Hospital.
Zhonghua Bing Li Xue Za Zhi. 1991 Sep;20(3):181-3.
Human lung giant cell carcinoma cell strains were implanted into the lungs of nude mice via an intrabronchial procedure. Tumorigenicity, invasion and metastasis of the xenografts, and their morphological features, including the findings by light and electronic microscopy and immunohistochemistry were investigated. Dissemination of xenografts within the airway (2/8), invasion to diaphragm (5/8), and development of bloody hydrothorax (5/8), were discovered. Metastasis via lymphatic and blood vessel routes as well as seeding metastasis occurred in all the tumor-bearing animals. The morphological features of xenografts were consistent with those of the parental giant cell carcinoma. These results indicate that lung cancer cell strains grow autonomously and the behavior of invasion and metastasis of the xenografts imitates more closely the clinical manifestation of lung cancer than their subcutaneous counterparts. Since microenvironment influences the biological behavior of the transplants, this model seemed to be more ideal for further investigation experimentally.
通过支气管内操作将人肺巨细胞癌细胞株植入裸鼠肺内。研究了异种移植瘤的致瘤性、侵袭和转移情况，以及它们的形态学特征，包括光镜、电镜和免疫组化检查结果。发现异种移植瘤在气道内播散（2/8）、侵犯膈肌（5/8）以及出现血性胸腔积液（5/8）。所有荷瘤动物均发生了经淋巴管和血管途径的转移以及种植转移。异种移植瘤的形态学特征与亲代巨细胞癌一致。这些结果表明，肺癌细胞株能自主生长，与皮下接种的异种移植瘤相比，其侵袭和转移行为更接近肺癌的临床表现。由于微环境会影响移植瘤的生物学行为，该模型似乎更适合进一步的实验研究。
