Schmalzing D, Nashabeh W, Fuchs M
PerSeptive Biosystems, Framingham, MA 01701, USA.
Clin Chem. 1995 Sep;41(9):1403-6.
We describe a competitive solution-phase immunoassay for serum cortisol determination that involves capillary electrophoresis (CE) combined with laser-induced fluorescence for separation and quantification. A polyclonal antibody preparation and fluorescein-labeled cortisol are used as assay reagents. 8-Anilino-1-naphthalenesulfonic acid was added to the serum sample during the assay to promote the release of cortisol from endogenous binding proteins. Conditions for the rapid separation of free and bound labeled antigen by CE were developed. Aspects of assay performance are evaluated in this report. The resulting assay protocol allows for the analysis of serum samples without extraction or other sample preparation steps.
我们描述了一种用于血清皮质醇测定的竞争性溶液相免疫测定方法，该方法涉及毛细管电泳（CE）结合激光诱导荧光进行分离和定量。使用多克隆抗体制剂和荧光素标记的皮质醇作为测定试剂。在测定过程中向血清样品中加入8-苯胺基-1-萘磺酸，以促进皮质醇从内源性结合蛋白中释放出来。开发了通过CE快速分离游离和结合的标记抗原的条件。本报告评估了测定性能的各个方面。所得的测定方案允许在无需提取或其他样品制备步骤的情况下分析血清样品。
