Koutny L B, Schmalzing D, Taylor T A, Fuchs M
PerSeptive Biosystems, Framingham, Massachusetts 01701, USA.
Anal Chem. 1996 Jan 1;68(1):18-22. doi: 10.1021/ac9508311.
An immunoassay performed using a microchip electrophoretic system is described. Separation and quantitation of free and bound labeled antigen in a competitive assay are carried out in channels micromachined into fused silica substrates. Such microchips are attractive because of their small size, ruggedness, and amenability to automated handling. The assay achieves the determination of cortisol in blood serum over the range of clinical interest (1-60 micrograms/dL) without the need for extraction or other sample preparation steps. The separation is performed in less than 30 s. Very high throughput is possible by operating the assay in multiple channels in parallel. These characteristics make microchip electrophoretic systems a promising technology for the rapid analysis of clinical samples.
描述了一种使用微芯片电泳系统进行的免疫测定法。在竞争性测定中，游离和结合的标记抗原的分离和定量在微加工到熔融石英基板中的通道中进行。这种微芯片因其尺寸小、坚固耐用且易于自动化处理而具有吸引力。该测定法无需提取或其他样品制备步骤即可在临床相关范围内（1 - 60微克/分升）测定血清中的皮质醇。分离在不到30秒内完成。通过在多个通道中并行操作该测定法，可以实现非常高的通量。这些特性使微芯片电泳系统成为临床样品快速分析的一种有前途的技术。
