Sukura A, Konttinen Y T, Sepper R, Lindberg L A
Dept of Anatomy, College of Veterinary Medicine, University of Helsinki, Finland.
Eur Respir J. 1995 May;8(5):701-8.
Our aim was to study histopathological changes in lung tissue at the light microscopic and ultrastructural level during recovery from immunosuppression and Pneumocystis carinii pneumonia. Male Wistar rats were immunosuppressed by per oral dexamethasone for 12 weeks to induce P. carinii pneumonia, after which dexamethasone was stopped. Recovery was monitored 1, 2 and 4 weeks after cessation of the immunosuppression. In immunosuppressed animals, CD4+ and CD8+ lymphocytes were both decreased in situ. CD8+ lymphocytes increased above control level at week one. Like CD8+ cells, the ED1+ macrophages increased rapidly in situ. This was accompanied by a progressively increasing migration (more transient for lymphocytes) of macrophages into bronchoalveolar fluid, associated with morphological signs of activation and phagocytosis and proliferation of type II pneumocytes. Bronchoalveolar lavage (BAL) fluid tumour necrosis factor-alpha (TNF-alpha) increased from subnormal levels to a 4 week peak, with an inverse correlation between TNF-alpha and cyst count (r = -0.626). Our observations suggest a sequence of changes characterized by an increase in CD4+ cells, accompanied by a more rapid and prolonged recruitment/activation of CD8+ cells, macrophages and type II pneumocytes.
我们的目的是研究免疫抑制及卡氏肺孢子虫肺炎恢复过程中肺组织在光镜和超微结构水平的组织病理学变化。雄性Wistar大鼠经口服地塞米松免疫抑制12周以诱导卡氏肺孢子虫肺炎，之后停用 地塞米松。在免疫抑制停止后1、2和4周监测恢复情况。在免疫抑制的动物中，原位CD4 +和CD8 +淋巴细胞均减少。CD8 +淋巴细胞在第1周增加至高于对照水平。与CD8 +细胞一样，ED1 +巨噬细胞在原位迅速增加。这伴随着巨噬细胞向支气管肺泡液中逐渐增加的迁移（淋巴细胞的迁移更短暂），伴有激活、吞噬和II型肺细胞增殖的形态学迹象。支气管肺泡灌洗（BAL）液肿瘤坏死因子-α（TNF-α）从低于正常水平增加到4周峰值，TNF-α与囊肿计数呈负相关（r = -0.626）。我们的观察结果表明一系列变化，其特征是CD4 +细胞增加，同时CD8 +细胞、巨噬细胞和II型肺细胞的募集/激活更快且持续时间更长。
