Dolbeare F
Biology and Biotechnology Program, Lawrence Livermore National Laboratory, University of California, Livermore 94551-9900, USA.
Histochem J. 1995 May;27(5):339-69.
Bromodeoxyuridine (BrdUrd), a thymidine analogue incorporated into DNA, can be quantified by fluorescent or chromophoric quenching of dyes bound to DNA or with antibodies to BrdUrd. These technologies have been used since the 1970s as tools for measuring DNA synthesis in isolated chromosomes and in cells and tissues. This paper is Part I of a three-part comprehensive review of the literature over the last 20 years (to the end of 1993) describing the histochemical methods for measuring BrdUrd in cells and tissues. Fixation, denaturation and staining procedures are compared for quantifying BrdUrd for microscopy and flow cytometry. Non-immunochemical methods related to the quenching of fluorescent DNA stains by BrdUrd are also described. Methods are described for the comparative assay of cell kinetic parameters by tritiated thymidine and bromodeoxyuridine. The multivariate BrdUrd/DNA assay of Ts and Tc, and a comparison of recent methods based on the single biopsy bivariate analysis of Tpot, is presented. Recent developments in the use of double halopyrimidine label to determine kinetic parameters are also reviewed.
溴脱氧尿苷（BrdUrd）是一种可掺入DNA的胸腺嘧啶类似物，可通过与DNA结合的染料的荧光或发色猝灭或使用抗BrdUrd抗体进行定量。自20世纪70年代以来，这些技术一直被用作测量分离染色体以及细胞和组织中DNA合成的工具。本文是对过去20年（至1993年底）文献的三部分全面综述的第一部分，描述了用于测量细胞和组织中BrdUrd的组织化学方法。比较了用于显微镜检查和流式细胞术定量BrdUrd的固定、变性和染色程序。还描述了与BrdUrd对荧光DNA染色的猝灭相关的非免疫化学方法。描述了用氚标记胸腺嘧啶和溴脱氧尿苷对细胞动力学参数进行比较测定的方法。介绍了Ts和Tc的多变量BrdUrd/DNA测定法，以及基于Tpot单活检双变量分析的近期方法的比较。还综述了使用双卤嘧啶标记来确定动力学参数的最新进展。
