Dolbeare F, Selden J R
Biology and Biotechnology Program, Lawrence Livermore National Laboratory, Livermore, California 94550.
Methods Cell Biol. 1994;41:297-316.
We have described several laboratory procedures for the immunochemical staining of the halopyrimidines, BrdUrd and IdUrd, in cell suspensions for flow cytometry and a method for staining histological sections on slides. Halogenated pyrimidine quantitation allows cell-cycle parameters, including total cell-cycle time, phase durations, and growth fraction to be determined. We have presented some flow cytometric data to demonstrate the use of these methods in determining bivariate BrdUrd/DNA histograms with CHO cells and in kinetic studies with the brown Norway rat myeloid leukemia model.
我们已经描述了几种用于在细胞悬液中对卤代嘧啶、溴脱氧尿苷（BrdUrd）和碘脱氧尿苷（IdUrd）进行免疫化学染色以用于流式细胞术的实验室程序，以及一种用于对载玻片上的组织切片进行染色的方法。卤代嘧啶定量可用于确定细胞周期参数，包括总细胞周期时间、各时相持续时间和生长分数。我们已经展示了一些流式细胞术数据，以证明这些方法在确定中国仓鼠卵巢（CHO）细胞的双变量BrdUrd/DNA直方图以及在棕色挪威大鼠髓系白血病模型的动力学研究中的应用。
