Nikas G, Drakakis P, Loutradis D, Mara-Skoufari C, Koumantakis E, Michalas S, Psychoyos A
Hôpital Bicêtre, Bâtiment INSERM Gregory Pincus, France.
Hum Reprod. 1995 May;10(5):1208-13. doi: 10.1093/oxfordjournals.humrep.a136120.
In 14 cycling women participating in an in-vitro fertilization (IVF) donation programme, we examined the timing of the 'nidation window' using as a stage-specific 'marker' the presence of fully developed pinopodes on the apical surface of the luminal uterine epithelium. Each woman received exogenous oestradiol from the second day of their cycle and progesterone starting on day 8 or day 15 of the oestrogenic treatment. The women underwent two biopsies during the same artificial cycle, on either days 6 and 9 or days 8 and 10 of the progesterone treatment. All patients to whom oestradiol was administered for 7 days prior to progesterone administration (n = 9), and two of the five treated with oestradiol for 14 days prior to the addition of progesterone, showed uterine pinopodes in either one or both biopsies. When present on a given day, pinopodes were at the same stage, developing, fully developed or regressing, showing that their total lifespan did not exceed 48 h. Fully developed pinopodes existed for 1 day only which may correspond to the short period of optimal endometrial receptivity observed in animal models. The timing of the presence of fully developed pinopodes varied from patient to patient, but these individual differences were not correlated with progesterone and oestradiol plasma concentrations. The brief duration of the nidation window and the observed individual variations in its timing suggest that the detection of uterine pinopodes could be a valuable tool for the prediction, on an individual basis, of the optimal date for successful egg replacement in IVF patients.
在14名参与体外受精（IVF）捐赠计划的骑自行车的女性中，我们以子宫腔上皮顶端表面完全发育的微绒毛为特定阶段的“标志物”，研究了“着床窗”的时间。每位女性从月经周期的第二天开始接受外源性雌二醇，并在雌激素治疗的第8天或第15天开始接受孕激素治疗。这些女性在同一个人工周期内进行了两次活检，分别在孕激素治疗的第6天和第9天或第8天和第10天。所有在孕激素给药前接受7天雌二醇治疗的患者（n = 9），以及在添加孕激素前接受14天雌二醇治疗的5名患者中的2名，在一次或两次活检中均显示有子宫微绒毛。当在某一天出现时，微绒毛处于相同阶段，即发育中、完全发育或退化，这表明它们的总寿命不超过48小时。完全发育的微绒毛仅存在1天，这可能与在动物模型中观察到的子宫内膜最佳接受性的短暂时期相对应。完全发育的微绒毛出现的时间因患者而异，但这些个体差异与孕激素和雌二醇的血浆浓度无关。着床窗的短暂持续时间及其时间上观察到的个体差异表明，子宫微绒毛的检测可能是一种有价值的工具，可用于个体预测IVF患者成功进行卵子置换的最佳日期。
