Grass D S, Saini U, Felkner R H, Wallace R E, Lago W J, Young S G, Swanson M E
DNX Biotherapeutics, Inc., Princeton, NJ 08540, USA.
J Lipid Res. 1995 May;36(5):1082-91.
Transgenic mice expressing both human apolipoprotein (apo) B and human cholesteryl ester transfer protein (CETP) have been developed. When fed a normal mouse chow diet, the apoB/CETP double transgenic animals had threefold higher serum CETP activity than humans and had human apoB levels that were similar to those of normolipidemic humans. When compared with nontransgenic mice, the total serum cholesterol levels in the female apoB/CETP transgenic animals were increased significantly. Serum HDL cholesterol levels were decreased significantly in both male and female apoB/CETP transgenic animals. The percentages of the total cholesterol within the HDL, LDL, and VLDL fractions of the apoB/CETP animals were approximately 30%, 65%, and 5%, respectively, similar to the distribution of cholesterol in the plasma of normolipidemic humans. Thus, by expressing both human apoB and human CETP, the lipoprotein cholesterol distribution in the serum of a chow-fed mouse was transformed into one that resembles a human profile.
已培育出同时表达人载脂蛋白（apo）B和人胆固醇酯转运蛋白（CETP）的转基因小鼠。当给apoB/CETP双转基因动物喂食正常的小鼠饲料时，其血清CETP活性比人类高三倍，且人apoB水平与血脂正常的人类相似。与非转基因小鼠相比，雌性apoB/CETP转基因动物的血清总胆固醇水平显著升高。雄性和雌性apoB/CETP转基因动物的血清高密度脂蛋白（HDL）胆固醇水平均显著降低。apoB/CETP动物的HDL、低密度脂蛋白（LDL）和极低密度脂蛋白（VLDL）组分中总胆固醇的百分比分别约为30%、65%和5%，类似于血脂正常的人类血浆中胆固醇的分布。因此，通过同时表达人apoB和人CETP，喂食饲料的小鼠血清中的脂蛋白胆固醇分布被转化为类似于人类的分布。
