Das R M, Ahmed M K, Mantsch H H, Scott J E
Department of Anatomy, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.
Mol Cell Biochem. 1995 Apr 12;145(1):75-9. doi: 10.1007/BF00925716.
Infrared spectroscopy which has traditionally been utilized by chemists and physicists for characterization and identification of the structural properties of chemical compounds is now becoming more relevant as a biodiagnostic tool. Recent reports suggest that arthritis and Alzheimer's disease can be diagnosed by using this technique. Changes associated with these diseases diagnosable with this technique are generally overt. In this study we have used 'Fourier transform infrared spectroscopy' (FT-IR) to analyze subtle changes in composition and structure of lipids and proteins in lung tissue, bronchoalveolar lavage and purified lamellar body fraction of mice exposed to methylmercury. Infrared measurements were made in attenuated total reflection mode using the Split Pea (Harrick Scientific Corporation, USA). Mice were treated with 4 doses of methylmercuric chloride (15 mg/kg body weight/dose), and control animals received an equivalent volume of physiological saline. Comparison of the control and experimental spectra revealed alterations in the intensities and frequencies of vibrational modes of lipids following methylmercury exposure. Results indicate that FT-IR spectral analyses may be a valuable tool for detecting subtle variations in biological components associated with drug exposure to lungs and, in particular may be very useful for assessing changes in bronchoalveolar lavage.
红外光谱法传统上一直被化学家和物理学家用于表征和识别化合物的结构特性，如今作为一种生物诊断工具正变得越来越重要。最近的报告表明，使用这种技术可以诊断关节炎和阿尔茨海默病。用这种技术可诊断出的与这些疾病相关的变化通常很明显。在本研究中，我们使用“傅里叶变换红外光谱法”（FT - IR）来分析暴露于甲基汞的小鼠肺组织、支气管肺泡灌洗物和纯化板层体组分中脂质和蛋白质的组成及结构的细微变化。使用Split Pea（美国哈里克科学公司）在衰减全反射模式下进行红外测量。给小鼠用4剂甲基氯化汞（15毫克/千克体重/剂）进行处理，对照动物接受等量的生理盐水。对照光谱和实验光谱的比较显示，甲基汞暴露后脂质振动模式的强度和频率发生了改变。结果表明，FT - IR光谱分析可能是检测与肺部药物暴露相关的生物成分细微变化的有价值工具，尤其对于评估支气管肺泡灌洗的变化可能非常有用。
