Del Poeta M, Barchiesi F, Arzeni D, Marinucci G, Scalise G
Institute of Infectious Diseases and Public Health, University of Ancona, Italy.
Mycoses. 1994 Nov-Dec;37(11-12):411-6. doi: 10.1111/j.1439-0507.1994.tb00391.x.
The drug concentration which inhibited 50% of growth (IC50), the lowest drug concentration at which growth was less than 30% of that in a positive control well (IC30), the visual minimal inhibitory concentration (MIC visual), were applied to study the effects of fluconazole, itraconazole, amphotericin B and flucytosine against 27 isolates of Cryptococcus neoformans by a broth microdilution technique. When the recommendations established by NCCLS Subcommittee on Antifungal Susceptibility Test were applied for the visual reading of the microplates, the results were comparable with those obtained by the turbidimetric method. No statistically significant differences between MIC visual and IC30 readings were observed with the azoles. There were, however, differences with amphotericin B and flucytosine. In absolute terms MICs of amphotericin B and flucytosine showed higher values than IC30s and IC50s.
采用肉汤微量稀释技术，应用抑制50%生长的药物浓度（IC50）、生长低于阳性对照孔30%时的最低药物浓度（IC30）、可视最小抑菌浓度（MIC可视），研究氟康唑、伊曲康唑、两性霉素B和氟胞嘧啶对27株新型隐球菌的作用。当按照美国国家临床实验室标准委员会抗真菌药敏试验小组委员会制定的建议对微孔板进行可视读数时，结果与比浊法获得的结果相当。对于唑类药物，MIC可视读数和IC30读数之间未观察到统计学上的显著差异。然而，两性霉素B和氟胞嘧啶存在差异。从绝对值来看，两性霉素B和氟胞嘧啶的MIC值高于IC30和IC50值。
