用111铟-奥克辛标记从循环血单核细胞体外生成的肿瘤细胞毒性巨噬细胞：一项体外评估
111In-oxine labelling of tumour-cytotoxic macrophages generated in vitro from circulating blood monocytes: an in vitro evaluation.
作者信息
Marienhagen J, Hennemann B, Andreesen R, Eilles C
机构信息
Nuklearmedizinische Abteilung, Universität Regensburg, Germany.
出版信息
Nucl Med Commun. 1995 May;16(5):357-61. doi: 10.1097/00006231-199505000-00007.
The labelling of ex-vivo activated macrophages with doses of 111In-oxine sufficient for gamma camera imaging does not damage cell viability or the functional competence of cells (secretion of tumour necrosis factor-alpha and interleukin-6). The mean labelling efficiency was about 84%. Release of 111In equivalent to 15% occurred over 24 h under cell culture conditions, indicating good stability of the radioactive cell label. 111In-oxine-labelled macrophages are a suitable tool for biokinetic studies of adoptive immunotherapy.
用足以进行γ相机成像的剂量的111铟-奥克辛对体外活化的巨噬细胞进行标记，不会损害细胞活力或细胞的功能能力（肿瘤坏死因子-α和白细胞介素-6的分泌）。平均标记效率约为84%。在细胞培养条件下，24小时内相当于15%的111铟释放出来，表明放射性细胞标记具有良好的稳定性。111铟-奥克辛标记的巨噬细胞是过继免疫疗法生物动力学研究的合适工具。
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