Mortelmans L, Verbruggen A, Malbrain S, Heynen M J, de Bakker C, Boogaerts M, de Roo M
Department of Nuclear Medicine, U.Z. Gasthuisberg, Leuven, Belgium.
Eur J Nucl Med. 1988;14(3):159-64. doi: 10.1007/BF00293542.
We have studied the influence of granulocyte labelling with commercially available 111In-oxine, tropolone (trop) or home made 111In-Mercapto pyridine (Merc) prepared by the method of Thakur (1985) on the cell structure by electron microscopy and on the cell function by enzymatic tests, random migration, chemotaxis, phagocytosis and bactericidal activity. The granulocytes were labelled with 400 microCi 111In-oxine in saline or 111In-trop or Merc in plasma. The effect of the chelating agents with and without addition of the tracer was studied (n = 4) with varying concentrations: 5-10 micrograms/ml oxine, 10-160 micrograms/ml trop and 1-4 micrograms/ml Merc. Chemotaxis and random migration were not affected by 111In-trop and clearly suppressed by 111In-oxine and Merc; the other tests were normal. The cell structure was disturbed by Merc. The labelling efficiency was excellent with oxine (90%), acceptable with trop (30%-80%) and poor with Merc (10%-25%). Without 111In, chemotaxis and random migration were normal up to a concentration of 80 micrograms/ml trop, 8.5 micrograms/ml oxine and 1 microgram/ml Merc. With addition of 111In, chemotaxis and random migration were unaffected up to 80 micrograms/ml by trop and markedly suppressed by Merc and oxine. It is concluded that labelling with 111In-trop assures intact cells.
我们通过电子显微镜研究了用市售的111铟-奥克辛、托酚酮（托普）或按照塔库尔（1985年）方法自制的111铟-巯基吡啶（Merc）标记粒细胞对细胞结构的影响，并通过酶学检测、随机迁移、趋化性、吞噬作用和杀菌活性研究了其对细胞功能的影响。粒细胞用盐水中的400微居里111铟-奥克辛或血浆中的111铟-托普或Merc进行标记。研究了添加和不添加示踪剂的螯合剂在不同浓度下的作用（n = 4）：5 - 10微克/毫升奥克辛、10 - 160微克/毫升托普和1 - 4微克/毫升Merc。111铟-托普不影响趋化性和随机迁移，而111铟-奥克辛和Merc则明显抑制趋化性和随机迁移；其他检测结果正常。Merc会扰乱细胞结构。奥克辛的标记效率极佳（90%），托普可接受（30% - 8%），Merc较差（10% - 25%）。在不添加111铟的情况下，浓度高达80微克/毫升托普、8.5微克/毫升奥克辛和1微克/毫升Merc时，趋化性和随机迁移正常。添加111铟后，托普在高达80微克/毫升时对趋化性和随机迁移无影响，而Merc和奥克辛则明显抑制趋化性和随机迁移。结论是用111铟-托普标记可确保细胞完整。
