哺乳动物散布重复（MIR）元件在哺乳动物基因编码和加工序列中的应用。
Use of a mammalian interspersed repetitive (MIR) element in the coding and processing sequences of mammalian genes.
作者信息
Murnane J P, Morales J F
机构信息
Laboratory of Radiobiology and Enviromental Health, University of California, San Francisco 94143-0750, USA.
出版信息
Nucleic Acids Res. 1995 Aug 11;23(15):2837-9. doi: 10.1093/nar/23.15.2837.
The mammalian interspersed repetitive (MIR) element was amplified in mammals 130 million years ago. The MIR element is at least 260 bp in length and is found in approximately 105 copies in the mammalian genome. We analyzed copies of the MIR element in the DNA of various mammals to determine its relationship to the structure and function of genes, in an attempt to identify specific uses of the MIR element within the mammalian genome. We found that alternative splicing within the acetylcholine receptor gene in humans takes place within the MIR element and results in the incorporation of part of the MIR element into the coding sequence of this gene. Furthermore, the polyadenylation signal (AATAAA) at the 3' end of four different mammalian genes is derived from the MIR element. These uses of the MIR element suggest that other regulatory sequences found within the mammalian genome originated from ancient transposable elements, many of which may no longer be recognizable.
哺乳动物散布重复（MIR）元件于1.3亿年前在哺乳动物中扩增。MIR元件长度至少为260碱基对，在哺乳动物基因组中约有105个拷贝。我们分析了各种哺乳动物DNA中的MIR元件拷贝，以确定其与基因结构和功能的关系，试图识别MIR元件在哺乳动物基因组中的特定用途。我们发现，人类乙酰胆碱受体基因内的可变剪接发生在MIR元件内，并导致该元件的一部分并入该基因的编码序列。此外，四个不同哺乳动物基因3'端的聚腺苷酸化信号（AATAAA）源自MIR元件。MIR元件的这些用途表明，哺乳动物基因组中发现的其他调控序列起源于古代转座元件，其中许多可能已无法识别。
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