一种使用可循环选择标记在酵母基因组中进行精确改变的方法。 - Suppr

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超能文献

A method for performing precise alterations in the yeast genome using a recycable selectable marker.

作者信息

Längle-Rouault F, Jacobs E

机构信息

Department of Molecular Genetics, Transgene SA, Strasbourg, France.

出版信息

Nucleic Acids Res. 1995 Aug 11;23(15):3079-81. doi: 10.1093/nar/23.15.3079.

DOI:10.1093/nar/23.15.3079

PMID:7659536

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC307157/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a7f/307157/ccc3e9af5d54/nar00015-0287-a.jpg

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本文引用的文献

A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae.一种在酿酒酵母中进行直接基因缺失的简单高效方法。

Nucleic Acids Res. 1993 Jul 11;21(14):3329-30. doi: 10.1093/nar/21.14.3329.

Mol Gen Genet. 1984;197(2):345-6. doi: 10.1007/BF00330984.

Transformation of intact yeast cells treated with alkali cations.经碱金属阳离子处理的完整酵母细胞的转化

J Bacteriol. 1983 Jan;153(1):163-8. doi: 10.1128/jb.153.1.163-168.1983.

A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains.一种基因破坏方法，可在构建多重破坏酵母菌株时重复使用URA3选择。

Genetics. 1987 Aug;116(4):541-5. doi: 10.1534/genetics.112.541.test.

Transformation of yeast with synthetic oligonucleotides.用合成寡核苷酸转化酵母。

Proc Natl Acad Sci U S A. 1988 Jan;85(2):524-8. doi: 10.1073/pnas.85.2.524.

The genetic control of direct-repeat recombination in Saccharomyces: the effect of rad52 and rad1 on mitotic recombination at GAL10, a transcriptionally regulated gene.酿酒酵母中直接重复序列重组的遗传控制：rad52和rad1对转录调控基因GAL10处有丝分裂重组的影响。

Genetics. 1989 Dec;123(4):725-38. doi: 10.1093/genetics/123.4.725.

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.一种用于在酿酒酵母中高效操作DNA的穿梭载体和酵母宿主菌株系统。

Genetics. 1989 May;122(1):19-27. doi: 10.1093/genetics/122.1.19.

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