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A method for performing precise alterations in the yeast genome using a recycable selectable marker.

作者信息

Längle-Rouault F, Jacobs E

机构信息

Department of Molecular Genetics, Transgene SA, Strasbourg, France.

出版信息

Nucleic Acids Res. 1995 Aug 11;23(15):3079-81. doi: 10.1093/nar/23.15.3079.

DOI:10.1093/nar/23.15.3079
PMID:7659536
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC307157/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a7f/307157/5f4e13aac83d/nar00015-0288-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a7f/307157/ccc3e9af5d54/nar00015-0287-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a7f/307157/5f4e13aac83d/nar00015-0288-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a7f/307157/ccc3e9af5d54/nar00015-0287-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a7f/307157/5f4e13aac83d/nar00015-0288-a.jpg

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Nucleic Acids Res. 1993 Jul 11;21(14):3329-30. doi: 10.1093/nar/21.14.3329.
2
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Mol Gen Genet. 1984;197(2):345-6. doi: 10.1007/BF00330984.
3
Transformation of intact yeast cells treated with alkali cations.经碱金属阳离子处理的完整酵母细胞的转化
Int J Mol Sci. 2023 Jul 26;24(15):11960. doi: 10.3390/ijms241511960.
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Identification of fungal dihydrouracil-oxidase genes by expression in Saccharomyces cerevisiae.通过在酿酒酵母中表达鉴定真菌二氢尿嘧啶氧化酶基因。
Antonie Van Leeuwenhoek. 2022 Nov;115(11):1363-1378. doi: 10.1007/s10482-022-01779-9. Epub 2022 Oct 14.
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Insertion orientation within the cassette affects gene-targeting success during ends-out recombination in the yeast Saccharomyces cerevisiae.在酿酒酵母中进行端出重组时,盒内的插入方向会影响基因靶向的成功率。
Curr Genet. 2022 Dec;68(5-6):551-564. doi: 10.1007/s00294-022-01246-y. Epub 2022 Jul 6.
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An Overview on Selection Marker Genes for Transformation of Saccharomyces cerevisiae.酿酒酵母转化的选择标记基因概述。
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